Why is it that always the mutant protein is synthesized?

In autosomal dominant disorders I understand that the expression of one allele is required for the proper functioning. In case of mutations why not the protein from the normal allele get...

31 December 2014 4,286 6 View

Cell Culture and confluency ?

10 November 2014 1,969 1 View

Protocol for culturing mouse iPSC

Can somebody share protocol for culturing mouse iPSC?

10 November 2014 8,812 3 View

Why is methionine always the first amino acid?

Why does tRNA recognize ATG as the first codon?

05 June 2014 2,240 11 View

Incorporation of ddGTP?

Why does ddGTP gets incorporated faster than other three ddNTPs during sequencing PCR?

04 May 2014 7,427 0 View

Is there any online tool to analyse frameshift mutation?

Is there any online tool to analyse frameshift mutation?

04 May 2014 2,086 6 View

Which is better for GC rich amplification: Betaine or DMSO?

Which is better for GC rich amplification?

03 April 2014 1,772 9 View

What is the difference between physical distance and genetic distance ?

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03 April 2014 9,714 5 View

How do some regions of the gene (or DNA) escape evolution and become conserved?

Thanks in advance.

01 February 2014 5,366 6 View

Can somebody share their GC rich kit protocol for PCR amplfication of difficult templates?

see above

01 February 2014 7,562 5 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What are some important techniques for protein induction and SDS-PAGE analysis?

Hello everyone. I am fairly new in protein chemistry field. I am trying to perform a protein induction hoping to purify my protein of interest using FPLC method. However, I am having trouble on...

03 March 2021 8,237 3 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View