Incorporation of ddGTP?

Bhairavi Srinageshwar @Bhairavi_Srinageshwar2

05 May 2014 0 8K Report

Why does ddGTP gets incorporated faster than other three ddNTPs during sequencing PCR?

Badges
Science topic

Similar topics
Cell Biology
Methods

More Bhairavi Srinageshwar's questions See All

Why is it that always the mutant protein is synthesized?

In autosomal dominant disorders I understand that the expression of one allele is required for the proper functioning. In case of mutations why not the protein from the normal allele get...

31 December 2014 4,454 6 View

Cell Culture and confluency ?

Usually how many days does it take for mesenchymal stem cells to attain 80-90% confluency?

10 November 2014 2,090 1 View

Protocol for culturing mouse iPSC

Can somebody share protocol for culturing mouse iPSC?

10 November 2014 8,970 3 View

Why is methionine always the first amino acid?

Why does tRNA recognize ATG as the first codon?

05 June 2014 2,367 11 View

Is there any online tool to analyse frameshift mutation?

04 May 2014 2,203 6 View

Which is better for GC rich amplification: Betaine or DMSO?

Which is better for GC rich amplification?

03 April 2014 1,881 9 View

What is the difference between physical distance and genetic distance ?

03 April 2014 9,849 5 View

Why does the gel turn cloudy white as it gets solidified during a Native PAGE protocol?

see above

01 February 2014 9,152 9 View

Can somebody share their GC rich kit protocol for PCR amplfication of difficult templates?

see above

01 February 2014 7,674 5 View

How do some regions of the gene (or DNA) escape evolution and become conserved?

Thanks in advance.

01 February 2014 5,482 6 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View