Why does a Nested PCR protocol amplify samples and not amplify positive control?

I have this problem. Today I used a new positive control of an isolated NC-1 strain in cell culture and the problem persists. I performed a serial dilution test to test the sensitivity of the...

08 December 2020 6,252 2 View

How to avoid the formation of traces and dimers in DNA gel electrophoresis?

07 December 2020 3,052 3 View

Is the pipetting order of the positive and negative controls in the PCR important to avoid in a possible contamination?

I have been watching bands form on my negative controls. I have already changed all reagents, optimized the hygiene of materials and the environment and increased care in pipetting.

07 December 2020 9,090 3 View

Best methodology to calculate food footprint in a university campus through students inquiring?

Hello, Considering sustainability and environment negative impacts of animal based products, I would like to calculate food footprint in a Portuguese university campus through students...

05 December 2020 9,098 3 View

How to standardize and obtain a good PCR reaction with samples with a low and variable amount of DNA?

I work with the detection of N. caninum DNA in milk samples in milk samples. The DNA concentrations in my samples range between 2-90 ng / ul. I would like to establish and standardize a final...

01 December 2020 8,792 3 View

Is there a database indicating the carbon and nitrogen storage capacity of vascular plants (in Europe)?

Hi everyone, I search a database or a review listing the capacity of vascular plants to storage carbon, nitrogen or other important chemical elements. I work actually on a mountain area (Swiss...

19 October 2020 8,596 3 View

How can we provide quality teaching in modular approach? And what are best practices on providing Remote Learning Support to learners?

I am so curious about how Philippines provide quality education using modular approach. Also, it is important to share best practices on how to provide feed back mechanisms on the learning...

19 August 2020 3,271 3 View

Does anyone have Abbink (1998)?

Hi people, i am looking for the following article: Abbink, O. A. (1998). Palynological Investigations in the Jurassic of the North Sea Region:(met Een Samenvatting in Het Nederlands): Proefshift...

02 August 2020 5,899 3 View

COVID-19, Human behaviour and wildlife impacts?

COVID-19 is causing a radical changes in Human behaviour around the world. Mandatory or voluntary isolation for months, suspension of the active management, etc.These changes will have positive...

24 March 2020 4,039 32 View

There are any component of gram + bacteria cell wall that can be used as a positive control?

Hello, I am going to evaluate the immunomodulatory effect of a gram positive probiotic bacteria in vitro and I need to use a positive control. I was thinking about LPS, but it's a component of...

11 March 2020 7,369 3 View

How to calculate the sample size in an interobserver variability study?

Hi I am conducting an interobserver variability study where we have 12 raters who are going to rate samples of lesions. They will rate up to 10 variables per sample. Although the size of the...

12 February 2021 7,276 3 View

How long and how could I best preserve gill tissue for histopathology?

I have a number of small bodied fishes that I want to extract gills from, so as to do morphological analayses and histopathology. The problem is: I have a lot more that I need to do the same work...

28 January 2021 6,091 3 View

How to make a model of trachea (human airway)?

I want to carry out flow analysis of air through tachea (human airway). How can I draw it in a Solidworks after taking a CT Scan image?

14 December 2020 5,031 2 View

In this sterile needle biopsy wet-mount, does the motility pattern fit with Oomycete zoospores?

We are examining tissue from a cluster of human and canine patients with a similar pattern of systemic illness of unknown cause. All members of the cluster have evidence of motile zoospore-like...

23 October 2020 4,480 3 View

How to do scoring for fat droplets in Histopathological sections?

I want to do scoring for macrovesicles and microvesiclular fat diposits in Histopathological Liver sections. Can anyone suggest me a software and discuss a suitable protocol to achieve this. I...

18 October 2020 9,727 2 View

How can you prepare rats synovium of knee joint for histopathology, H and E and special stains?

Tissue processing techniques in particular..

06 September 2020 5,701 3 View

How can I keep the Sigmoid Function Formula during Cross Validation of Logistic Regression?

I have conducted a binary logistic regression. There are 294 lesions and logistic regression can separate them as benign and malignant. For this purpose three of the study parameters produced a...

09 August 2020 4,489 1 View

Where can I find medical image data of healthy subjects for machine learning?

Hi, I want to implement a binary classifier (lesion yes/no) with the DeepLesion dataset. Therefore I also need ct images of healthy subjects because the DeepLesion dataset only includes images...

29 July 2020 9,764 14 View

Where may I find a common ultrasonic image database for breast lesion's detection and segmentation, please give some suggestions?

to detect and segment breast lesion for breast cancer in ultrasonic breast imgae

27 July 2020 8,338 3 View

Histological scoring of acute inflammation in Trachea?

Hello, Does anyone have a detailed protocol for H&E histological scoring of acute inflammation in particularly "trachea"? Thank you. Nazli

05 July 2020 7,932 3 View