Gromacs 2020: how to make distance restrains for 2 atoms in different protein chains?

Sorry, I think it is rather simple problem to solve, but I am stuck on it. I have 2 protein chains, so I have 2 *.itp files for them that are loaded in topol.top. What should I do to use distance...

01 December 2020 1,496 1 View

Different Ct in qPCR with three sets of reference genes primers (each ref.gene is a single copy per genome), with the same template - is it normal?

I am trying to build up 3 standard curves for 3 sets of primers for different reference genes, in order to later use one with the best efficiency. (for SYBR green qPCR, gene copies number...

27 February 2018 8,313 3 View

QPCR with SYBR green - should the amplicons (GOI and reference) be the same length ?

Do I understand right: when running qPCR with SYBR green detection, it is important that two amplicons (GOI and reference gene) should be of the same lenght ? Once It is supposed to be relative...

07 February 2018 4,678 5 View

How do you assess the efficiency/ rate of mutagenesis, when you plate your culture on the selective plate (e.g. canavanine or 5-FOA) ?

After the chemical or UV mutagenesis, the yeast culture is supposed to be plated on the selective medium with canavanine or 5-FOA in order to assess the efficiency of mutagenesis. The questions...

04 January 2018 4,813 3 View

How to do non-water solvent parametrization for Molecular Dynamics?

Currently I need to parametrize some simple F and Cl containing solvents to use them in Gromacs. The problem is that common force fields do not contain atom-types I need, so while understanding...

18 October 2016 7,283 3 View

Could I expect any quantitative difference in EDX results, if the analysis is performed on SEM/TEM samples (same steel and area)?

INCA (Oxford Instruments), X-Max 80 Cr-Ni stainless steel

16 May 2016 7,436 9 View

How to solve the structure of a complex in crystallography?

22 February 2016 2,615 3 View

Could anyone help me with continuous casting modelling in Abaqus ?

11 October 2014 246 1 View

Diabetes data set for ANN

Hello. Can anyone help me with some useful data for my Master's diploma? I am making an Artificial Neural Network for prediction diabetes. I know about some open data sets like Pima Indians,...

01 January 1970 6,480 2 View

How to avoid fake journal articles published under your authorship without your permission?

01 January 1970 726 4 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Mediation path analysis - Can I run two seperate models?

I am wanting to run a serial mediation model with 2 x IVs, 2 x MVs, and 3 DPs (all continuous). Is it problematic to run 2 different models for each IV, keeping the MVs and DPs the same in each...

27 February 2021 5,681 3 View

SEM or Multiple Regression for an analysis without Latent Variables?

Hello, I have three hypothesis to test, all with the same IV. I am testing if the effect of the IV on the first DV is mediated by awe. I am then measuring whether the effect of the IV on a...

26 February 2021 8,399 5 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View