Recombinant protein concentration for an in vitro assay?

Hello everyone, I would like to observe the effects of a recombinant protein on in vitro cultured cells. For this reason, I need to know a concentration at which recombinant proteins can be used...

28 May 2020 5,304 3 View

How long does it last the labelling with PKH26 on EVs?

Hello everyone, I have a concern about the EVs staining with the lipophylic dye PKH26. I would like to stain EVs and then inject them in order to assess their distribution within the liver. My...

12 May 2019 8,621 3 View

How to stain exosomes with PKH26?

Hello people, I would like to stain EVs and see whether, once stained, they are captured by cells or they interact with cell surface. I was looking to the manifacturer's instructions and I...

02 December 2018 6,486 2 View

Is there a method to check for RNA integrity/quality that does not require to run an agarose gel nor to use Bioanalyzers?

Hi everybody, I would like to assess the quality of my RNA before performing further analyses (RT-qPCR) but I do not have access to Bioanalyzers like the Experion or Agilent 2100 Bioanalyzer. I...

06 February 2018 927 2 View

Software for generating synthetic stream flows?

04 May 2017 4,738 1 View

Could someone tell me if Glycoblue (ThermoFisher) can affect Nanodrop readings of RNA samples?

I use Glycoblue to dye my RNA pellets deriving from cytosolic or nuclear RNA (concentrations ranging from 150 to 600 ng/uL) and everything is pretty fine with the absorbance ratios from...

03 May 2017 5,959 4 View

How can I prepare salivary simulated fluid?

23 November 2016 2,141 3 View

Which is the relationship between Collaborative Innovation and Open Innovation?

We are developing an interdisciplinary study, which relates Communication discipline and Innovation theory. For that reason, we want to know, how does this relationship work? Do you know papers...

04 May 2016 3,897 0 View

Are you familiar with path analysis with distance matrices?

I would like to perform a path analysis but I only have 4 distance matrices. First, can I really do it? And what software should I use?

12 April 2015 3,468 3 View

Are you familiar with path analysis with distance matrices?

I would like to perform a path analysis but I only have 4 distance matrices. First, can I really do it? And what software should I use?

03 April 2015 1,429 3 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

Are there any open-source datasets containing physiological information about exercises to be used for an OR-model?

I have just recently started a new "weekend project" in addition to my master's studies and I am looking for a data-set. I would like to use some Operations Research to design an optimal gym...

22 February 2021 8,023 1 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

Is the box plot of climatic indices better illustrate from daily data or annual data?

Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...

19 February 2021 9,742 2 View

Would you please recommend a function in R software that could be used with FUNGuild output to merge different rows that have similar guilds?

Dear colleagues, I have an output of FUNGuild tool (ASVs abundance in different samples and assigned to guilds) and would like to merge the ASVs that had similar ecological guilds using R...

16 February 2021 4,292 7 View

What is/are the best ML classifier(s) for Sentinel2 images?

Hi I am trying to segment a sentinel2 image. At this stage, I want to run a binary classifier that assigns each pixel to either farm or non-farm pixel. For this purpose, I have 4 10m bands...

15 February 2021 3,596 9 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View

Can I modify vermiculite or perlite by adding nutrients to control a certain situation?

I need to assimilate a determining condition for the plant. However, I need to ask you if it is possible to change the properties and concentration of plant nutrients in this material like perlite...

04 February 2021 8,616 11 View

Is there any publication about the effect of basketball and volleyball on body height?

Many people believe that some stretching sports with vertical jump such as basketball and volleyball can increase the body height. On the other hand, others do not agree with this argument. The...

02 February 2021 281 6 View