If you co-stain a sample with two dyes with Emax=561 and Emax=633, can you still distinguish between the two dyes? Or is there too much bleedover?

I need to stain tissue for octopamine and synapsin together and need to avoid wavelengths between 450-500 as my tissue gives too much autofluorescence in this range. I cannot go lower than 400 so...

21 March 2018 8,923 4 View