I want to detect Ire1-alpha in fixed, adherent human cells. Could you recommend a reference for which you validated signal specificity with a knock-down or knock-out of Ire1? Many thanks!
We have only worked with proteins downstream of the IRE1, (XBP1, GRO78 y 94. Ire antibodies are generated for wester blot, but in our experience they are not good for immuno.