Does anyone know if A549 cancer cell line express cGAS-STING?

27 April 2023 1 10K Report

I was wondering if A549 cell line express cGAS and STING or if these genes are mutated in this cell line

Thank you

Hello Valentin Estevez

A549 cancer cell line does not express cGAS and STING. You may work with H358 cell line which is a non-small cell lung cancer (NSCLC) cell line. This cell line expresses cGAS and STING.

You may want to refer to the paper I have attached below for your reference. (see Fig 1A, 1C and 1F).

https://tlcr.amegroups.com/article/view/46144/html

Best.

Badges
Science method

More Valentin Estevez's questions See All

Can you recommend an equation to predict the maximum slide thickness in function of the propagation distance, slope angle and slide properties?

We would like to predict the maximum slide thickness at a particular location along a laboratory slope from an initially triangular-shaped dry granular slide released as a dam break. Can you...

13 May 2024 6,316 0 View

Which is your experience with immersive virtual reality in medical education?

I would like to know what is your experience with immersive virtual reality in training programmes for nurses or doctors. If you have tried any immersive virtual reality simulator please send me...

03 March 2024 5,056 10 View

Seeking Suggestions for Finance Thesis Topic,do you have any ideas?

I am currently in the process of selecting a topic for my finance thesis, and I am seeking your valuable insights and suggestions. My area of interest lies in value investing strategis,corporate...

15 January 2024 5,576 7 View

Can you recommend an analytic model for the runouts of dry granular slides in function of the internal and basal friction angles?

We are running lab tests of dry granular slides at different scales and want to quantify the effect of the internal and basal friction angle on the maximum runout.

03 November 2023 9,097 1 View

Is it possible to use large amplicon sequencing ( e.g. plasmidsaurus) to detect large deletions after CRISPR/CAS9 use?

We want to complement our indels study in primary cells after CRISPR/CAS9. We are looking for a cheaper way to identify large deletions in our targeted loci. Normal Tide/ice analyses only...

01 November 2023 1,710 1 View

Upper Cretaceous planktonic and benthic foraminifer from Costa Rica, a good recognition?

Hi everyone. As I am not a foraminifera specialist, and quite new in studying upper Cretaceous deposits, I have a question for you. I "recognized" in these 4 pictures: - Rugoglobigerina rugosa -...

15 August 2023 3,994 1 View

Any idea of what this Upper Cretaceous foraminifera form could be ?

This foraminifera is the only specimen found in a Maastrichtian small isolated carbonate platform in Costa Rica, Central America.

02 August 2023 2,012 6 View

How to prepare highly concentrated emulsion of magnetic fluid in water?

Dear colleagues Recently, I decided to reproduce the approach for the synthesis of magnetite/polystyrene microparticles via emulsion polymerization developed by dr. Gu lab. I used this...

12 June 2023 3,409 2 View

Could you please the two profiles, since I am the same scientis Christian Valentin, IRD?

/profile/Christian-Valentin contains 7 publications /profile/Christian-Valentin-4 contains 334 publications

06 May 2023 5,487 1 View

RAW ultrasound data in PWD (pulse wave doppler) mode?

Can anyone provide a recommendation as to which medical ultrasound device allows to RAW RF data in PWD mode? The most common example I see give access to RAW data in B-mode. But I'm are...

21 February 2023 6,937 6 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View