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Questions related from Valentin L'Hôte
My protocol requires the establishment of clonal cell lines through the expansion of single cell clones sorted in individual wells by FACS. This is notoriously challenging for fibroblast cell...
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I want to detect Ire1-alpha in fixed, adherent human cells. Could you recommend a reference for which you validated signal specificity with a knock-down or knock-out of Ire1? Many thanks!
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I want to use KX2-391 (tirbanibulin) to inhibit Src in cultured fibroblasts. Is it known whether treatment with this compound results in decreased or increased phosphorylation of Src? I would like...
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I came across conflicting protocols online about how lentivirus should be thawed properly before transduction. I saw either to thaw it rapidly at 37°C then keep it on ice, or to thaw it slowly on...
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