Valentin L'Hôte

5 Questions 3 Answers 0 Followers

Questions related from Valentin L'Hôte

How uniform should be the sizes of DNA fragments in a Nanopore sequencing library?

I have a DNA sample which is a mixture of fragments ranging in size from 100bp to 15kbp. I am interested in performing Nanopore sequencing on this sample, hopefully retaining information on both...

09 August 2024 3,352 1 View

How to stimulate clonal growth of fibroblasts after single-cell sorting?

My protocol requires the establishment of clonal cell lines through the expansion of single cell clones sorted in individual wells by FACS. This is notoriously challenging for fibroblast cell...

24 October 2022 8,527 2 View

Can you recommend a monoclonal anti-Ire1 antibody validated for immunofluorescence?

I want to detect Ire1-alpha in fixed, adherent human cells. Could you recommend a reference for which you validated signal specificity with a knock-down or knock-out of Ire1? Many thanks!

15 February 2022 7,883 2 View

What is the effect of KX2-391 (tirbanibulin) on Src phosphorylation in cultured cells?

I want to use KX2-391 (tirbanibulin) to inhibit Src in cultured fibroblasts. Is it known whether treatment with this compound results in decreased or increased phosphorylation of Src? I would like...

02 April 2021 8,479 0 View

Should lentiviral libraries be thawed rapidly at 37°C or slowly on ice?

I came across conflicting protocols online about how lentivirus should be thawed properly before transduction. I saw either to thaw it rapidly at 37°C then keep it on ice, or to thaw it slowly on...

23 September 2019 1,704 3 View