I have generated a figure "Gene distribution by conservation" from 20 genomes of bacteria. Can someone explain how to interpret correctly?

I have generated a figure "Gene distribution by conservation" from 20 genomes of bacteria. Can anybody explain how to interpret it correctly? Thank you in advance

11 January 2021 3,346 2 View

Problem with PCR using plasmid as template?

Hi I am trying to do a PCR for a 1kb fragment using a plasmid of 11kb as a template. But unfortunately on the gel, the band is as large as the plasmid, that is 11kb. Would anyone know that that...

20 October 2020 7,631 3 View

How can I get access to the travaux preparatories of international conventions related to Intellectual Property Rights?

For instance, the 'Historical Archives' section of the 'Audio Visual Library of the International Law' website gives access to the negotiation documents related to various instruments of...

07 June 2020 3,135 3 View

How to do primary cell culture from tumor tissue and injection to mice?

Dear Karen, I am aiming to isolate primary cells from freshly resected tumor tissue from consented patient and injecting those cells to mice. We have failed two times doing so, because the cells...

15 January 2020 8,262 2 View

What are the miRNA profiling alternatives?

I am interested in looking at miRNA signature may be for 10-15miRNAs, but amount of input RNA is limited. qRTPCR is not realistic, since it can provide only one or two miRNA data. Nanostring and...

26 April 2019 9,862 3 View

Rod to spore shaped 'particles' in cell cultures, bacterial contamination?

Last week I had consecutive contaminations of some of my mammalian primary cells alone. It started with the cultures turning turbid after a day or two of incubation. I wiped down the entire...

11 February 2019 5,127 8 View

Is WatchPAT a home sleep monior?

Is WatchPAT from Itamar medical a home sleep monitor or sleep apnea monitor? Can it be used to generate the ground truth for sleep staging? Any reliable references of the use of the device as a...

11 December 2018 1,313 2 View

How to interface arduino to Visual basics 2015?

can someone suggest me any website for visual basics coding or else give tell me how can I learn it quickly

30 November 2018 3,130 1 View

What is the difference between Subject specific and Epoch specific clustering?

Many of the machine learning algorithms use k-means clustering to improve the clasification performance based on human physiological signals. However, while some studies uses a subject specific...

08 November 2018 2,624 1 View

What is the advantage of using statistical techniques before feature selection using wrapper method?

I have seen literature reporting the use of statistical techniques like Mann-Whitney U test and Cohen's D effect size to identify most suitable subset of features for a classifcation problem. But...

15 September 2018 6,687 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View