please help me with the question asked above .
I have generated a figure "Gene distribution by conservation" from 20 genomes of bacteria. Can anybody explain how to interpret it correctly? Thank you in advance
11 January 2021 3,346 2 View
Hi I am trying to do a PCR for a 1kb fragment using a plasmid of 11kb as a template. But unfortunately on the gel, the band is as large as the plasmid, that is 11kb. Would anyone know that that...
20 October 2020 7,631 3 View
For instance, the 'Historical Archives' section of the 'Audio Visual Library of the International Law' website gives access to the negotiation documents related to various instruments of...
07 June 2020 3,135 3 View
Dear Karen, I am aiming to isolate primary cells from freshly resected tumor tissue from consented patient and injecting those cells to mice. We have failed two times doing so, because the cells...
15 January 2020 8,262 2 View
I am interested in looking at miRNA signature may be for 10-15miRNAs, but amount of input RNA is limited. qRTPCR is not realistic, since it can provide only one or two miRNA data. Nanostring and...
26 April 2019 9,862 3 View
Last week I had consecutive contaminations of some of my mammalian primary cells alone. It started with the cultures turning turbid after a day or two of incubation. I wiped down the entire...
11 February 2019 5,127 8 View
Is WatchPAT from Itamar medical a home sleep monitor or sleep apnea monitor? Can it be used to generate the ground truth for sleep staging? Any reliable references of the use of the device as a...
11 December 2018 1,313 2 View
can someone suggest me any website for visual basics coding or else give tell me how can I learn it quickly
30 November 2018 3,130 1 View
Many of the machine learning algorithms use k-means clustering to improve the clasification performance based on human physiological signals. However, while some studies uses a subject specific...
08 November 2018 2,624 1 View
I have seen literature reporting the use of statistical techniques like Mann-Whitney U test and Cohen's D effect size to identify most suitable subset of features for a classifcation problem. But...
15 September 2018 6,687 2 View
I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...
21 February 2021 5,379 2 View
I have cloned two domains of a gene into two bacterial vectors using cut and paste cloning with different RE sites. I checked the putative clones by colony PCR and Restriction Digestion (fallouts...
18 February 2021 6,736 4 View
Hello We are working on a PCR product with an approximate size between 5000 and 7000 bp, we want to use it as a positive internal control, since we have sequenced it and it corresponds to the...
17 February 2021 2,896 3 View
Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...
14 February 2021 2,610 1 View
Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...
14 February 2021 2,310 1 View
Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...
12 February 2021 1,386 3 View
Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...
08 February 2021 7,285 1 View
07 February 2021 2,006 1 View
Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...
04 February 2021 6,051 8 View
01 February 2021 9,576 3 View
