I have generated a figure "Gene distribution by conservation" from 20 genomes of bacteria. Can someone explain how to interpret correctly?

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Problem with PCR using plasmid as template?

Hi I am trying to do a PCR for a 1kb fragment using a plasmid of 11kb as a template. But unfortunately on the gel, the band is as large as the plasmid, that is 11kb. Would anyone know that that...

20 October 2020 7,631 3 View

How to calculate thermal stress in cylinder if water is flowing inside the cylinder?

How to calculate thermal stress in stainless steel cylinder if water is flowing inside the cylinder?

14 October 2020 905 2 View

How extract and purify extracellular proteins expressed in E. coli?

Hello everyone, I am having trouble isolating and purifying an extracellular protein which I have expressed in E.coli. I have induced my protein at 0.5 mM IPTG by incubating it at 37 degrees for...

02 October 2020 8,223 3 View

Systematic Review and Meta-analysis?

Hi, I am planning to conduct a systematic review and meta-analysis since very long. However, found difficulty in literature search and statistical analysis. Please anyone advice me any easy way...

11 July 2020 7,868 13 View

Can I use the same research instrument for collecting data from two different populations?

Can I use the same questionnaire to collect data from health as well educational sector employees to measure their level of job satisfaction ?

02 July 2020 7,480 1 View

Can anyone help me finding good impact factor journals to submit uninvited review paper on postharvest chilling injury in fruits.?

I have tried submitting my review paper in relevant journals, but most of them only accept the review paper invited by the editor of the journal

28 June 2020 4,371 5 View

How can I get access to the travaux preparatories of international conventions related to Intellectual Property Rights?

For instance, the 'Historical Archives' section of the 'Audio Visual Library of the International Law' website gives access to the negotiation documents related to various instruments of...

07 June 2020 3,135 3 View

How to calculate the sample size for a clinical study where the exact disease prevalence is not available?

While planning clinical study like RCT on diseases like COVID-19, how can we calculate the sample size when exact disease prevalence is not available

01 May 2020 5,079 3 View

How to do primary cell culture from tumor tissue and injection to mice?

Dear Karen, I am aiming to isolate primary cells from freshly resected tumor tissue from consented patient and injecting those cells to mice. We have failed two times doing so, because the cells...

15 January 2020 8,262 2 View

What are the major problems and challenges in social science research?

What are the major problems and challenges in social science research and let us proposed comprehensive solution accordingly. The considerable known challenges are development of...

27 September 2019 1,757 13 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Mixed ANOVA - help?

I'm currently working on a piece which asks to conduct an ANOVA - looking at the effect of sporting group (2 levels), and exercise trials (two trials with six levels). I've computed this all...

27 February 2021 9,626 4 View

How to Interpret Partial Eta Squared Numbers For a Repeated Measures Anova?

I have obtained Partial Eta Squared Numbers through Two-Way Mixed Anova Analysis. My results for different constructs are: .105, .135, .038, .068, .061 respectively. I wanted to know, whether...

24 February 2021 7,122 1 View

Interpretation of Goodman - Kruskal Gamma squared?

Dear colleages, Eta squared is dominantly used in reflecting the explaining power the same manner as a squared partial correlation coefficient (R2p ) from multiple linear regression: as the...

22 February 2021 7,801 3 View

How to use Remote sensing and GIS applications for mapping the available medicinal plants within the locality/municipality?

Hello, I'm very interested in mapping and managing the conservation of the Philippine traditional medicinal plants, in which I think is very helpful in the field of medicine for further analysis...

21 February 2021 374 5 View

Interpretation of a fluorescence Emission spectrum ?

Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...

20 February 2021 1,291 3 View

In CDA analysis we have three stages, viz., description , interpretation, and reproduction. Why have all CDA studies neglected the third stage?

18 February 2021 6,483 3 View

How can I interpret a ratio variable?

Dear colleagues, I am running a regression in which I have the following regression FoodExp = 67.04 DepRatio +.....e My interest is in the interpretation of the coefficient on the dependency...

16 February 2021 2,058 2 View

How do you interpret theoretical vs. experimental reaction orders with respect to a reactant?

I recently came across a paper focusing on the hydrodeoxygenation of oxygen-containing compounds. The paper linearized the rate equation in order to determine the experimental reaction order with...

14 February 2021 8,254 2 View