Anusha Pulavarty

5 Questions 1 Answers 0 Followers

Questions related from Anusha Pulavarty

Protein residues after performing TCA Acetone precipitation method?

Hello... could someone please suggest me if the plant proteins extracted using TCA Acetone method do completely dissolve in the SDS containing buffer or do they still have some residues? If they...

05 October 2021 9,438 3 View

Hello..Could someone please suggest me the best procedure for protein isolation from root & leaf tissues of tomatoes for SDS-PAGE and 2D analysis?

I am studying the effect of certain soil health products on plant protein expression. I am in particular interested on plant defence related proteins and pathogenesis related proteins. Could...

20 September 2021 9,088 3 View

Can you please tell me at which wavelength should we detect trehalose while estimating using HPLC?

Wavelength is not mentioned anywhere.

27 April 2014 9,269 2 View

What is the most appropriate protocol for estimation of sucrose and trehalose content in plant tissues?

I need to estimate these in salt treated plant samples.

27 March 2014 8,538 3 View

Can someone tell me what the maximum absorbance of glycine betaine is?

In most papers it is reported that maximum absorbance of glycine betaine as 365nm, but even after several repetitions I am getting its maximum absorbance at some other wavelength (i.e 200-220nm).

26 March 2014 8,565 2 View