What is the best way to identify brain mast cells in mice?

09 March 2023 0 3K Report

I've been trying to identify mast cells in mouse brains with Toluidine blue staining but unable to get the results. What would be the best possible method to identify mast cells in a rodent's brain?

Badges
Science topic

Similar topics
Eutheria
Rodentia
Muridae
Murinae
Mice

More Anusha Govindula's questions See All

Fish physiology and biochemistry journal ?

No response from fish physiology and biochemistry journal after a year of peer review. Journal editorial team is not responding even for Request for withdrawal of our manuscript. We are struck...

25 June 2024 8,918 0 View

"prot:A:MET1:HN1 and prot:A:MET1:HN1 have the same coordinates" Error While Performing Flexible Docking?

After selecting the residues of the protein for flexible docking, when I click on "Choose Torsions In Currently Selected Residues", under the " Flexible Residues" tab, I get this error:...

28 December 2022 2,376 1 View

How can we identify Cohesive end fragment and Blend end fragment using Restriction enzyme ?

please help me with the question asked above .

27 July 2022 7,120 0 View

What is the procedure to design a plate fin heat exchanger with phase change in one of the streams?

Any recommendations on books would be really helpful.

26 March 2022 2,840 2 View

Difference between L-Cysteine Hydrochloride anhydrous and monohydrate?

Hello everyone, I am culturing pseudomonas aeruginosa in BUP2 media using cysteine HCl. What is the difference it will cause if I use L-Cysteine Hydrochloride monohydrate instead of L-Cysteine...

12 January 2022 6,854 3 View

From where can I get an article on consumer preferences regarding cosmetics in Germany and India?

I am writing my master thesis on consumer, preferences and brand loyalty and its marketing strategies in Germany and India. It is a comparative study so i wanted some articles regarding the same.

07 December 2021 847 2 View

Protein residues after performing TCA Acetone precipitation method?

Hello... could someone please suggest me if the plant proteins extracted using TCA Acetone method do completely dissolve in the SDS containing buffer or do they still have some residues? If they...

04 October 2021 9,446 3 View

Hello..Could someone please suggest me the best procedure for protein isolation from root & leaf tissues of tomatoes for SDS-PAGE and 2D analysis?

I am studying the effect of certain soil health products on plant protein expression. I am in particular interested on plant defence related proteins and pathogenesis related proteins. Could...

19 September 2021 9,109 3 View

I have generated a figure "Gene distribution by conservation" from 20 genomes of bacteria. Can someone explain how to interpret correctly?

I have generated a figure "Gene distribution by conservation" from 20 genomes of bacteria. Can anybody explain how to interpret it correctly? Thank you in advance

11 January 2021 3,668 2 View

Problem with PCR using plasmid as template?

Hi I am trying to do a PCR for a 1kb fragment using a plasmid of 11kb as a template. But unfortunately on the gel, the band is as large as the plasmid, that is 11kb. Would anyone know that that...

20 October 2020 7,866 3 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,081 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,156 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 639 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,706 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,246 3 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,891 1 View