Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Can anyone please help me in processing SAR SLC data to generate interferogram using SNAP?

I have two scenes of the study area, one top and the other bottom. As per the tutorials and papers published, the step include coregistering the master sand slave images. However, when i try to...

24 February 2021 6,372 1 View

Any natural extract (that is easily available) or any drug combination triple which i can use against gram negative?

I'm working on gram negative i want to use any natural extract (plant extract) or triple drug combination against its biofilm formation.

14 February 2021 1,403 3 View

Regarding to presentation of pairwise comparison of data?

22 January 2021 8,648 1 View

Can I use hcl to adjust ph rather than glacial acetic acid?

In alkaline lysis solution3 acetic acid should be used to adjust pH according to protocol. Can I use hcl instead of glacial acetic acid? Will there be any change in composition of solution?

08 January 2021 7,764 3 View

What is the best method for plasmid dna extraction??

I want to extract plasmid dna from bacterial culture guide me what will be the most appropriate method.

05 January 2021 1,118 14 View

How can we find crop transpiration (Tr) value from ETo values in Aquacrop model?

it like how we can get Tr value separately from ETo values in Aquacrop

20 December 2020 5,817 3 View

How to find load / force acting on the top face in ansys workbench?

The image attached is the representation of my application. This is an assembly of three items (top plate, bottom plate and the 5 load cells). The bottom plate is fixed and a load of 40 Kg is...

15 December 2020 1,145 6 View

Unlikely condition in the water inundation area after the cyclone?

I have done flood mapping with Sentinel 1 data for 3 districts of India (West Bengal) i.e. North Pargana, South pargana and East Medinipur. North and South Pargana are showing increasing trend in...

11 December 2020 4,714 3 View

How to calculate Area-Weighted Average Temperature at outlet in Ansys?

Suppose a fluid is flowing between two parallel plates (and exchanging heat) for 100-time steps. So, there is an area-weighted Average temperature at every 10 steps. How can I write a UDF to...

29 November 2020 6,240 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View