Om Prakash

10 Questions 10 Answers 0 Followers

Questions related from Om Prakash

Can any one share recipe of Red blood lysing buffer for lateral flow assay test development?

I am developing lateral flow assay in my lab. I want to know the recipe of red blood lysing buffer. In addition, I am also interested knowing about the most suitable washing buffer, blocking...

11 October 2017 6,992 2 View

Can anyone let me know how goat anti-human IgM binds with human IgM?

Actually, I want to know that how anti-human IgM bind with human IgM. I want to know the specific reaction. So, I will appreciate if anyone could share their knowledge.

28 January 2017 1,344 4 View

Immunosesnor vs ELISA. Can any one let me know distinguish advantages of immunosensor over ELISA?

I have read a lot of articles and review articles regarding immunosensor, in the articles researcher compares the sensitivity of immunosensor vs ELISA. But other than this, I could not any...

28 January 2015 2,364 9 View

Which brand is good for dengue polyvalent antigen?

I want to perform dengue IgM ELISA manually. Could anyone suggest to me a good brand for antigen and antibody for use in this test?

07 June 2014 3,632 4 View

Can any let me know the simple way to introduce a NH2 (amine) group on carbon electrode?

Actually, I want to pretreat my carbon working electrode with amine group for antibody immobilization by using the carboxylic group of antibodies.

21 February 2014 4,371 2 View

How to remove inhibitors from serum samples?

When I perform immunoassay using a commercial antigen(purchased from abcam) it works but when I use a positive serum sample it does not work. Can any one let me know how to remove these inhibitors...

06 August 2013 1,069 3 View

Can anyone let me know what is the optimal incubation time and potential while performing amperometry using Hrp conjugated body with TMB and H2O2?

Actually, I want to detect my protein using hrp labelled antibody on SPCE by using amperometry techniques. I am using hydrogen peroxide as substrate with mediator TMB. I want to know that what...

25 February 2013 5,764 1 View

Can anybody let me know how to introduce carboxylic group on screen printed carbon electrode?

I want to immobilize the capturing antibody on a carbon working electrode using a covalent technique with EDC/NHS, but prior to that I have to introduce COOH group on the carbon electrode. So, can...

03 February 2013 6,673 5 View

Can any body let me know what kind of charge is available at screen printed carbon electrode? Does it contain any functional group as well?

I am looking for surface chemistry of screen printed carbon electrode. I want to know what kind of functional group and charge is available on carbon surface. In addition, I want to introduce...

10 November 2012 2,517 2 View

Can we use the concentration of IgG below 3.8mg/ml for Peroxidase labeling Kit?

According to Roche company's protocol we have to use 3.8mg-4.2mg/ml IgG concentration for HRP-antibody conjugation. But the amount of antibody is too high to manage. Is it possible to reduce the...

12 June 2012 2,307 2 View