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Questions related from Om Prakash
I am developing lateral flow assay in my lab. I want to know the recipe of red blood lysing buffer. In addition, I am also interested knowing about the most suitable washing buffer, blocking...
11 October 2017 6,965 2 View
Actually, I want to know that how anti-human IgM bind with human IgM. I want to know the specific reaction. So, I will appreciate if anyone could share their knowledge.
28 January 2017 1,328 4 View
I have read a lot of articles and review articles regarding immunosensor, in the articles researcher compares the sensitivity of immunosensor vs ELISA. But other than this, I could not any...
28 January 2015 2,320 9 View
When I perform immunoassay using a commercial antigen(purchased from abcam) it works but when I use a positive serum sample it does not work. Can any one let me know how to remove these inhibitors...
06 August 2013 1,041 3 View
Actually, I want to detect my protein using hrp labelled antibody on SPCE by using amperometry techniques. I am using hydrogen peroxide as substrate with mediator TMB. I want to know that what...
25 February 2013 5,747 1 View
I want to immobilize the capturing antibody on a carbon working electrode using a covalent technique with EDC/NHS, but prior to that I have to introduce COOH group on the carbon electrode. So, can...
03 February 2013 6,659 5 View
According to Roche company's protocol we have to use 3.8mg-4.2mg/ml IgG concentration for HRP-antibody conjugation. But the amount of antibody is too high to manage. Is it possible to reduce the...
12 June 2012 2,278 2 View
