SDS Gel Electrophoresis: I am looking to get sharper bands

I wanted 5uG in each lane. For Lane 2 I had a concentration of 1.08mg/mL = 1.08ug/uL. 5ug x 1uL/1.08ug = 4.63uL. I mixed (4.63uL x 3) with the same volume of laemlli sample buffer to get a total...

31 January 2013 3,176 7 View