Platelet is a nucleated cell and PI is specific nuclei binding DNA dye. So I was wondering whether PI works in differentiating dead and apoptositc platelets.
Hi Mallikarjun, I'm afraid you are mistaken. Human platelets do not have a nucleus.
You can't distinguish dead and apoptotic platelets, You can distinguish them on the basis of their functionality and by parameters that are collectively termed "platelet storage lesions".
Hi Mallikarjun,
I agree with Dr. Steingrimur Stefansson, PI can not be used on platelets we used it on platelet precursor -megakaryocyte's and not on platelet directly
Thank you...Yes I knew that platelet is a anucleated cell...in the question it was a typing mistake...That's why I was confused whether to use or not
Hi Mallikarjun, no problem. The most common way to distinguish platelet function by FACS is to use fluorescent annexin V.
http://scholar.google.com/scholar?q=annexin+platelets+FACS&btnG=&hl=en&as_sdt=1%2C21
Hello,
Just keep in mind that in platelets AnnexinV won't give precise info about apoptosis. It detects also (and primarily) platelet activation. Article Annexin V binding to platelets is agonist, time and temperat...
Best.
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