Am working on Coding sequence of the bacterial plasmid and need to calculate the codon stabilization coefficient. So can anyone suggest a procedure/tool/software to calculate it.
Why is CS655 soil moisture sensor reading Soil water content (SWC) as 6999 or -6999
26 March 2024 1,468 0 View
can any body tell me what is the maximum compressive strength of the natural knee cartilage? presently I am working on hydrogel (artificial implant)for cartilage regeneration and replacement so...
06 August 2023 10,019 0 View
We have a system for pulsed laser deposition. While operating, we are having vacuum-related issues. Without the use of a laser, we can initially maintain a vacuum of 100 pa, which will remain...
29 January 2023 9,713 3 View
I want to perform compression and tensile test for a hydrogel sample for biomedical application. What should be the dimensions of the sample? Can anyone provide ASTM protocol for compression testing?
02 January 2023 335 2 View
Need information regarding the cultivars grown across India
13 December 2020 7,389 1 View
Why the values of the HOMO-LUMO gap is different for ground-state geometry obtained from DFT and first excited state geometry obtained from TD DFT. What they signify physically?
09 July 2020 6,328 8 View
i.e., Are they dependent??
08 June 2020 8,207 4 View
Tourism is important industry that has very high potential for employment and self employment. The development of this industry on sustainable basis demands green concepts and principles to be...
21 January 2019 5,344 0 View
Patenting an work or Idea
31 December 2018 8,202 4 View
Does microinjection itself alter the developing embryo? if So, what percentage of embryos might get affected due to microinjection?
18 March 2016 6,274 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?
29 July 2024 3,229 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View