I am trying to form amyloid beta 1-42 oligomers for i.c.v injection in rats. As per WB. Stine protocol use of Hams F12 culture media is recommended but I am facing availability issues. Can I use DMEM instead of Hams F12 phenol free culture media?
NO. It doesn't work. Stick with the Ham's. Use the Ham's with phenol if you have to, but make sure you have a blank before spec'ing any assay with color.
You can't get Ham's without phenol these days except dry powder.
Thank you for the response Iris Lindberg .
As rightly said, it is difficult to obtain phenol free Hams F 12 media except dry powder.
Do you recommend the use of phenol free or phenol containing Hams F 12 for amyloid beta oligomerization?
Both types of Ham's work. The only problem is the color if you are doing something spectrophotometric. But you can correct for it with a blank.
Dear Iris Lindberg ,
I intend to use the amyloid beta 1-42 incubated in Hams F 12 containing phenol in rats via ICV route. Therefore, for my sham control I am planning to use Hams F 12. Is this fine? since I wasn't able to find any literatures to support it.
I have no experience with injecting cell culture medium into mice, just PBS, but I think it should be fine since it is isotonic.
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