I am trying to develop an efficient and an effective AD model using Wistar rats.I require suggestions as to which model may be the best one to mimic AD pathology in rats. The one that comes to my mind is Amyloid Beta 1-42 i.c.v injection. But a wide range of Amyloid Beta 1-42 are available so would like to seek advice from the fellow researchers who might have used it in their work. Also would like to know if any other better routes of administration are available or not, as i.c.v is an invasive one.
Note: I am looking out for non- transgenic models of AD.
Hi Sairaj Satarker ,
There are numerous established and well-substantiated ways to induce AD in rodents.
I have used a number of them in our different studies, and I found out that the main principle is to outline what you are looking for as the rationale or hypothesis of your study first. You may want to perform a research to study systems neuroscience as we have recently done to study the role of cholinergic projections to cortical layers on somatosensation in healthy and AD rats.
In this case and since one may want to have a broad view based on histological and neuroanatomical findings, they may induce AD by chemical or electrical lesion in a certain brain region depending on the neurotransmission system that they intend to study.
You may want to induce a non-specific AD neuropathology to see the overall memory deficits and to study learning dysfunction in rats using behavioral or electrophysiological and optogenetics probes. In this case, you may want to use local injections of AB 1-42:
Article Chronic ghrelin administration restores hippocampal long‐ter...
You can also use agents such as streptozotocin as we did to induce AD-like neuropathology in rats.
Dear Sairaj Satarker ,
I also agree with Bahman Sadeghi , that your hypothesis can influence the choice of the model.
However, since you are looking for non-invasive and non-transgenic models, I would suggest using Aluminum chloride (AlCl3) or D-galactose models. Various studies have used these models to mimic AD-neurotoxicity. Some useful Links:
AlCl3:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6571973/
https://www.ncbi.nlm.nih.gov/pubmed/26897372
D-galactose:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5568415/
I hope this helps, Good Luck.
Dear Satarker,
You can use d-galactose and aluminium chloride to induce AD-like pathology in rats. https://doi.org/10.1016/j.biopha.2018.04.152. You can send me email for the complete version of the article. [email protected]
Thank you.
Thank you Bahman Sadeghi and Firas Hasan Bazzari for the vivid description.
Thank you Musa Samaila Chiroma for your valuable inputs.
I intend to study neuroinflammation caused in AD and w.r.t that want to simulate AD conditions in rats.
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