I am using cobalt chloride for HIF1 induction in cancer cell lines. I prepare 10-20 ml of stock solution, but only 50-100 ul is used at time. The rest of the solution is discarded. Is there a way to store the stock solution?
I'm also planning to use CoCl2 solution for HIF induction in cell lines. Can you please tell me how did you made your stock solution? I read mixed opinions were some people diluted de CoCl2 in destiled water while others used DMSO. What did you used? Thank you.