Shrikant Pradhan

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Questions related from Shrikant Pradhan

Can we store 25mM stock solution of CoCl2.6H2O at 4 degree or -20 degree C?

I am using cobalt chloride for HIF1 induction in cancer cell lines. I prepare 10-20 ml of stock solution, but only 50-100 ul is used at time. The rest of the solution is discarded. Is there a way...

05 May 2016 3,460 5 View

What internal controls can be used while comparing the gene expression in normoxic versus hypoxic conditions?

I am working on lung cancer cells lines and comparing the expression of certain set of genes in normoxic versus hypoxic conditions.

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What precautions should be taken to prevent photobleaching of phalloidin-rhodamine at higher magnifications?

I am using cancer cell lines for my study. At lower magnifications of 20 and 40X, the stain is not affected even for long exposure time. However, at 60X, using oil, the stain bleaches in a matter...

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