What pH condition required to coat APTES on silica coated Fe3O4?

It's a hydrolysis reaction, which may produce by either acid or base-catalyzed reaction. so can anybody help me with this, which condition is suitable for this reaction?

26 February 2021 1,760 8 View

What condition required for coating of Fe3O4@SiO2 by using APTES?

I tried coating Fe3O4@ SiO2 by using APTES in 5% water-ethanol but it is not coated. Kindly suggest a solution for it. Thanks

24 February 2021 5,992 4 View

How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View

Is it possible to design digital all pass filter for specific frequency range of the system?

I would like to know, Is it possible to design digital all pass filter for specific frequency range of the system to alter the phase of the system in that specific range of frequency? If yes how...

26 November 2020 1,402 5 View

In some CP patch design the polarization changes for LHCP to RHCP, What should be the possible reasons?

I have designed a dual print dual cp antenna, identical patch on both side of the substrate (placed side by side), on analysis, it is found it is showing LHCP in one band and RHCP in another band....

04 August 2020 5,136 3 View

In dual band antenna why the gain in upper band decreases?

In some printed antenna designs, generally, the gain in the upper band is larger than the lower band. However, in some cases (in my design having printed circle on both side of the substrate like...

03 August 2020 5,802 5 View

How to cap the maximum amount of reinforcement addition to make a polymer to be thermally conductive?

Addition of reinforcement with very high thermal conductivity (ex. graphene) would enhance the thermal conductive value of the developed polymer composites. How would one cap the maximum weight...

21 June 2020 9,303 11 View

We are facing rotten problem in kesar mango near mouth of it. What may be reason for that?

We are doing natural farming of kesar mango. This year we are facing rotten problem in mango near mouth of it. Last year it seems good. For this reason we are facing problem in selling if it....

02 June 2020 5,277 7 View

In COVID-19 modeling estimates for India by CDDEP, Johns Hopkins, and Princeton, what decideds peaking and decay?

COVID-19 modeling estimates for India have been presented by a team of researchers affiliated with CDDEP, Johns Hopkins, and Princeton. The results described in...

28 March 2020 1,623 2 View

Up to what temperatures can nCovid-19, novel corona virus survive?

The question does not require much explanation. Interested to know if there is some study on overall pattern of temperature range of permanent inactivity of various viruses and bacteria of all...

10 March 2020 8,749 44 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View