Dear all,
I am using Is eFluor™ 670 eBioscience die (Invitrogen 65-0840-85) to stain NK cells. The datasheet indicates that it binds to any cellular protein containing primary amines and that it can be used for long term tracking of labeled cells. I am wondering if cells might loose the staining if cells die.
Basically, I do not achieve a uniform staining of all NK cells with Fluor™ 670 eBioscience, since some of them are much brighter than others. Also, I am wondering whether in some conditions I might not be visualising that many labelled NK cells in case cells lose the die upon cell death. Could anyone shed some light on this?
I am trying to track NK cells over 24h-48h (microscopy) and I would need a die giving a reliable staining for the cells so any help will be much appreciated.
Also, could Hoechst staining be an option? I know it perfectly stains all my NK cells, but does anyone know if Hoechst staining night affect NK cell functionality?
Many thanks in advance for your time and help.
Kind regards,
Ainara
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