Nehal Ghoneim

6 Questions 5 Answers 0 Followers

Questions related from Nehal Ghoneim

Can we induce DNA damage in cancer cell line using a UV light in the laminar flow hood?

Hi, I want to induce DNA damage using the UV light in the laminar flow hood? I inquire is that possible and what is the required duration? Thanks,

13 September 2023 3,105 1 View

Neuroblastoma SH-SY5Y can be used as positive marker for neuronal differentiation?

Can I use Neuroblastoma SH-SY5Y as a reference for the expression of neuronal markers ( Nestin - B III tubulin - GFAP - MAP2) to confirm the neuronal differentiation by real-time quantitative PCR.

04 September 2018 1,680 4 View

Best Gel documentation system ?

Please, I don't know which is better Bio rad Chemi DOC xrs+ or Azure Biosystems™ c280 for protein and DNA gel applications !

03 April 2018 6,501 4 View

Electroporation of cripr cas9 by gene pulser?

Kindly I need to do transfection for canecr cell lines by Crispr cas 9 from santa cruiz (downregulation and overexpression) , I wonder if cuvette 0.2 will work with it as I don't have 0.4 and the...

29 March 2018 3,550 2 View

Size of electroporator cuvette size for mammalian transfection ?

I am trying to transfect SNU449 cell line by GFP plasmids using genepulserX Bio rad electroporator. I wonder if I can use .4 cm cuvette and voltage needed ! and what is the cell size of SNU449 !

22 March 2018 7,980 3 View

Does any body know whether MicroPulser™ Electroporator works for transfection of mammalian cells ?

I am trying to do transfection of SNU 449 hepatocellular carcinoma cells by GFP plasmids using Bio Rad micropulser electroporator ! according to its protocol it nables safe and reproducible...

14 March 2018 8,377 3 View