Can we store tissue in RNAlater for miRNA isolation ?

More Alka Khera's questions See All

I would like to do a systematic review on management of Pulmonary Regurgitation for a repaired TOF patient ?

completed

21 October 2023 727 4 View

Can we get membrane bound proteins using Trizol?

RIPA is the best way to get the membrane bound proteins right? But has anyone tested Trizol isolated proteins to see if we get membrane proteins?

03 October 2023 2,306 3 View

What is the difference between a cloning vector and Expression vector?

how we can differentiate them on the basis of expression of the gene?

17 May 2023 5,950 3 View

Can somebody help calculating enzyme kinetics parameters with GC-MS method?

I am working on an enzyme and calculating the amount of product formed with GC-MS method. So I have calculated the amount of product frormed in uM from the area under curve of product and known...

05 September 2022 6,191 1 View

pEC86 plasmid map?

Hi everyone, Does anyone know which inducer is needed to overexpress pEC86 plasmid ? Has anyone used this plasmid to overexpress cytochromes and has details? Thanks

21 June 2021 8,500 3 View

Recombinant plant protein not giving its expression in yeast (Saccharomyces cerevisiae)?

Hello everybody I have a problem with protein expression and the purification of genes from a plant source in yeast. I cloned the gene successfully in pYES2.1 through TOPO TA cloning kit...

21 April 2021 6,066 17 View

The protein interaction from MST did not give the proper saturation curve?

I am studying protein-protein interactions and from MST, I observed the binding during binding check but I cannot get a full saturation anywhere with binding affinity tests. I tried changing the...

16 February 2021 7,164 4 View

Can we have a model where A affects B which affects C but B is not a mediator?

Please suggest any papers in Information Management area that have the model where A affects B which affects C but B is not a mediator? What is this relationship called?

13 February 2021 5,635 3 View

What does it mean if a model is non significant ?

I am doing statistical analysis to find the effects of different treatments on samples. Results are coming insignificant at 1% level of significance. What does it mean ?

17 March 2020 7,614 2 View

Do you think theoretical thematic analysis can fit with a critical realist ontology constructivist epistemology?

I am conducting qualitative research for my doctorate in counselling psychology, exploring humanitarian workers’ experiences of morally conflicting events, through the lens of Litz et al.’s (2009)...

15 February 2020 1,882 6 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Which modeling software and force field suitable for miRNA-mRNA molecular simulation?

Hello everyone, I am currently working on miRNA- mRNA interaction study. Further, I would like to do MD simulations for the miRNA-mRNA models. For the Simulation, I have modelled this complex...

24 June 2024 3,507 2 View

Which is the best small Extracellulare Vesicles (sEVs) concentration to obtain enough RNA for miRNA analysis?

Hello to everyone, I'm trying to extrtact total RNA from sEVs. I made a first experiment starting from 2x10^10 sEVs quantified by NTA but the RNA obtained was very low (under 20ng/ml) Do you have...

18 June 2024 2,799 4 View

What is the best way to synthesize cDNA of miRNA; using poly A kit or stem loop PCR?

Recently, I have been trying to quantify the expression of miRNA. I have extracted the miRNA. However, I am not clear about using the cDNA synthesis kit usually there are two kits providing by...

16 June 2024 9,723 2 View

Extraction RNA with miRVANA kit?

Hello everyone, I would like to know if any of you ever tried to assess the yeld of RNA extraction with the mirVana™ miRNA Isolation Kit, without phenol from ThermoFisher. I am experincing some...

06 June 2024 5,715 4 View

How to extract RNA from powdered plant tissue stored in RNAlater?

My research group has samples of plant tissue that were pulverized in ln2 using a mortar and pestle before being weighed into tubes and stored in RNAlater at -20 degrees for a few months before...

28 May 2024 7,274 0 View

I need guidance how to design primers for miRNA?

The size miRNA is 89 bps and the primers I have designed (using traditional method for designing gene primers) should give an amplified product of 71 bps. But I am getting product of higher...

23 May 2024 7,570 3 View

Should the target miRNA level decrease after the miRNA inhibitor transfection when checked with qPCR??

I have a question regarding target miRNA expression after transfection with antisense miRNA (miRNA inhibitor). I understand that the expression of the target miRNA will increase after mimic...

20 May 2024 5,920 4 View

Webserver for identifying activation and repression of TFs, genes, and miRNAs?

Hello. Is there any webserver where I can input a set of transcription factors, genes, and miRNAs, and it can provide me with statistical data on how TFs either activate or repress genes and...

18 May 2024 5,848 1 View

Does anybody face problems with total RNA isolated after miRNA mimic and inhibitor transfection?

Hello all. I have recently been trying to isolate total RNA after transfection of miRNA mimics and antisense inhibitors in mammalian cells. However, the RNA isolated through the trizol method...

13 May 2024 4,935 7 View

Anupam Mittal

Hi. I suggest you to keep tissues in RNA later at 4 degree overnite then transfer them to -20 n then to deep freezer -80. MicroRNAs are very stable even at RT without any reagent but later u can use ur tissues for larger RNAs

Brian Scott Comer

It will work for large and small RNAs. Just follow the recommended protocol.