Can we have a model where A affects B which affects C but B is not a mediator?

Please suggest any papers in Information Management area that have the model where A affects B which affects C but B is not a mediator? What is this relationship called?

13 February 2021 5,239 3 View

What does it mean if a model is non significant ?

I am doing statistical analysis to find the effects of different treatments on samples. Results are coming insignificant at 1% level of significance. What does it mean ?

17 March 2020 7,357 2 View

Do you think theoretical thematic analysis can fit with a critical realist ontology constructivist epistemology?

15 February 2020 1,397 6 View

Recombinant cyt c expression in e.coli?

I overexpressed soluble cytochrome c in e.coli. After Ni-NTA. I am getting two protein bands on SDS-PAGE - the one which matches my theoretical protein molecular weight 40 kda is having a fainter...

03 January 2020 1,679 2 View

Hello Researchers ! what is actual use of absolute summability in BIBO stability of electrical system ? what is deep concept of the following image?

Please help me in understanding the deep concept of the relation of BIBO stability and absolute summability.

30 September 2017 9,759 1 View

How can we find fractional atomic coordinates and isotropic displacement parameters for a crystalline sample?

30 September 2016 5,694 3 View

Would THLE2 cells grow well in DMEM?

Currently working with THLE2 cells but don't have any experience with these cells. Do they only grow in BEBM media or they would grow well in DMEM as well?

22 August 2016 9,815 7 View

Why does grain size increase with increasing dopant concentration in case of Fe doped BaTiO3 ceramics ?

In Fe doped BaTiO3 ceramics, both crystalline size and grain size will increase with increasing Fe concentration. Can anyone explian ?

11 January 2016 3,755 3 View

Has anyone done TUNEL assay for apoptosis in suspension cell lines?

I am getting v high background in control cells, using colorimetric kit.

22 March 2015 901 2 View

Do prostate cancer cell lines (PC3, DU145, LnCap) or normal prostate cells RWPE-1 secrete IL-17 or IL-23?

PC3, DU145, LnCap secret TNF-a, IL-6, IL-8, IL-12 upon LPS/LTA stimulation. Do these cell lines also secret IL-17 or IL-23 (basal levels or stimulated)? I need help.

16 February 2015 325 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Which miRNA arm is a right target for analysis, 3p or 5p?

I'm working on selecting mouse miRNAs to analyze and stumbling upon one confusing issue, some of the papers do not specify which arm they analyzed and they just state something like " miR-34a was...

16 January 2021 3,792 4 View

Is negative controls necessary for western blotting?

I am trying to confirm my miRNA target by western blotting. I have a ds mimic and a ss inhibitor of my miRNA. Is it necessary to use negative controls for mimic and inhibitor? If yes, how can I...

09 January 2021 2,320 3 View

Can I predict miRNA by knowing the mRNA target?

Hello! I am trying to predict in silico the miRNA by knowing the target. Most of published research starts by knowing the miRNA and predicts the target using online tools. Are you familiar with...

31 December 2020 7,433 5 View

What are the possible drawbacks of using miRNA sponge to enhance the specificity of gene expression?

I am searching for miRNA repeats put between WPRE and 3'LTR of my lentivirus, in order to enhance the specificity of transgene expression in human neuron cells. But if clinically translation of...

26 December 2020 9,921 2 View