How to calculate total soluble sugars or total carbohydrates by anthrone method?

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Chinaza Godswill Awuchi

Anthrone reagent: Dissolve 2g of Anthrone in 1 litre of concentrated H2SO4. Use freshly prepared reagent for the assay (ii) Glucose stock solution: 200μg glucose per mL distilled water. Note: Can include other carbohydrates of the same concentration if desired. boiling water bath for 10 minutes. https://www.iitg.ac.in/biotech/MTechLabProtocols/Carbohydrate%20estimation%20by%20Anthrone.pdf

Check https://www.iitg.ac.in/biotech/MTechLabProtocols/Carbohydrate%20estimation%20by%20Anthrone.pdf