Can we add tris buffer powder while preparing dehydrated culture media as buffering agent?

More Madhura Kadam's questions See All

How can we add External Lock-in Amplifier for Seiko SPA 400? I want to measure amplitude(1st harmonic ) vs DC bias sweep?

I need to add external lock-in amplifier but I am confused with connection.

29 January 2024 6,172 0 View

How we can calculate Vcpd after getting surface potential image ain KPFM ?

I have this image from surface potential how I can get CPD value from this data?

24 January 2024 1,795 4 View

Can anyone suggest an effective live cell plasma membrane dye for MDCK II TISSUE ?

Hi all, I'm working on MDCK II cell line where I grow the cells on PDMS substrate into a dense tissue (about 5mm diameter in size). I have been trying out different plasma membrane dyes for my...

14 January 2024 9,842 0 View

How can we calculate the amount of shelling precursor for the synthesis of core-shell nanoparticles to get particular shelling thickness?

We have the diameter of the core nanoparticles.

19 November 2023 3,113 3 View

What are the impurities of semaglutide?

What are the impurities formed during the synthesis and purification of semaglutide. kindly define,give their RRT and molecular mass also.

28 October 2023 8,130 2 View

How to obtain onset limit cycle oscillations in a Rijke tube?

with the boundary conditions of inlet as mass flow rate and outlet as pressure, the oscillation of pressure with flow rate is continuously diverging and it does not sustain limit cycle oscillation.

27 October 2023 1,385 0 View

How to decide the practical applicability of our prepared super-capacitor electrode material for specific application in portable electronics?

Hello, Researchgate community, I have one query regarding deciding whether our electrode material is suitable for practical energy storage applications. When we prepare and characterize...

22 October 2023 6,027 3 View

How can I decide ISI and jitter intervals for rapid stimulus presentation in fMRI picture memory encoding task?

The brain activity during individual images will be analyzed post-hoc for correctly recognized/ forgotten pictures during retention a few days later. Currently, we have 150 images; 3 seconds for...

17 September 2023 7,836 0 View

What exactly happens during the process of narrowing of C:N ratio?

How Carbon breaks, why N ratio remains same?

16 August 2023 9,957 4 View

How to decide the concentration need to induce oxidative stress in isolated mitochondria of rat brain by using either rotenone & sodium nitroprusside?

I'm trying to induce oxidative stress by using rotenone and SNP sodium nitroprusside in isolated mitochondria of rat brain. This is to study GSH assay from control (Mitochondria) group in...

11 June 2023 6,247 2 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

04 August 2024 6,961 3 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Panduranga Rao

Is it for animal cell culture? The question is not clear.

Tris is not a preferred buffer for animal cell culture media. Sodium bicarbonate/ CO2 system or Hepes are generally used buffering systems.

Saif Al-Mofty

You could use HEPES with humidifier if you don't want to use the CO2 incubator

Minakshi Prasad

one can use HEPES buffer and Sodium Bicorbonate for buffereing . Another alternate could be Phosphate buffer saline (PBS).