Say Fungus A gives 500 bp and Fungus B gives 550 bp is it an absurd result?
In short the answer is Yes. The sequence lengths can vary between these primers as such the weight as well.
How was molecular weight determined? The answer to that question will impact the answer to your question.
More directly, molecular weight /= sequence length. Two different things.
ITS1 and ITS4 amplifies the Internal Transcribed Spacer region. The sequence and the sequence length for this region will be different for different organisms.
For example, Fungus A may have sequence: tgggtcattgattcac while Fungus B may have sequence: cgtaacgaatgccattgcaccatgcc. Note that 1) the nucleotide sequence are different for both fungi and 2) the length of sequence is different for both fungi. The difference in characteristics are important in the process of identifying what fungus that is. Therefore, it is normal if the size of the band you obtain from amplification has different size/molecular weight/basepair for different fungi.
- Badges
- Science method