I am growing astrocytes from the striatum of adult (70 days) male Sprague Dawley rats. I use laminin+poly-D-lysine to coat T75 flasks on which the astrocytes could attach. Of late, the astrocytes are unable to adhere to the flask surface and are dying off. I have tried using a new coating solution, and new plates but nothing seems to help.
Could anyone please help!!
Hi Samrat Bose
I use T75 flask with 20 ml of poly-D-lysine (PDL) at a concentration of 50 μg/ml in cell culture grade water for 1 hr at 37 °C in the CO2 incubator. Following coating I was the flask with sterile water or PBS. The astrocyte attachment works quite well for me.
Ref: Article Isolation and Culture of Mouse Cortical Astrocytes
Thanks
Samir
Hi Samrat
No I use P-0 to P-2 mice for isolation and culture of astrocytes.
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