Can the blot be stored overnight directly after after ponceau staining ?

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Jeff Overton

You try blocking overnight at 4C, then Abs the next day RT

Blaise Robin

If you leave the blot in washing buffer ON maybe it will be better to do again the saturation step the next day. Does anyone think the same ? :)

Rajan Singh

Thank you people for the response. I stored it overnight with 1x TBST and then did the washing with the milk buffer the next day.

Yung-Feng Lin

Usually, I remove the liquid and store it at -20C. The blotting process can be continue anytime later. It works well for me.