Rajan Singh

4 Questions 5 Answers 0 Followers

Questions related from Rajan Singh

How can I use the NF KAPPAB Inhibitor BAY 11-7085 ?

I am looking at the NF-Kappab inhibition in fibrobalsts. In one of the references from the inhibitor data sheet i found that the cells were incubated with the BAY-11-7085 inhibitor at a...

21 October 2015 6,179 5 View

Can E-Caherin be detected using Flow Cytometry in Spheroids over time ?

I am working on Cancer cell spheroids in moment and plan to detect the inhibitory effects of Fibroblasts on Cancer cells when incubated over a time period of 24 hours, 48hours and 72 hours. Does...

06 August 2015 5,346 3 View

What time differences should i use to study the NF-KappaB p50 translocation to the nucleus ?

I have currently used 4 time periods for looking at my cells for the NF-kappab activation: 5,10,15 and 20 minutes. Looking at the western blot during these specific time points, the increase in...

19 May 2015 3,069 4 View

Can the blot be stored overnight directly after after ponceau staining ?

is it possible to do the rest of the protocol of western blotting the next day and store the blot until the next day ?

06 May 2015 4,795 4 View