Rajeev Meora

6 Questions 32 Answers 0 Followers

Questions related from Rajeev Meora

Use of publicly available genomes/metagenomes require authors’ permission?

Dear all, I am currently engaged in a metagenomic research project and intend to incorporate several publicly available metagenomes into my analysis alongside my own data. These publicly...

09 October 2023 5,489 8 View

Greeting Everyone, Number Of OTUs needed from NGS data ?

Recently, I have sequenced few samples (seawater source) using Illumina MiSeq platform and good quality sequences (70,000-1,20,000) are taxonomically assigned using QUIIME software pipeline....

12 June 2018 3,744 13 View

Depth needed for sequencing obtained through Next Generation Sequencing (NGS) ?

Hii, Greeting Everyone Currently i am working on Next Generation Sequencing, will anyone suggest me the depth/coverage needed for a sequence obtained through any platform of NGS particularly...

11 January 2018 4,437 5 View

Greeting Everyone, Which hyper variable region of 16S rRNA gene is suitable for microbial community assessment in seawater through NGS ?

I am working on microbial diversity and planing to send samples for Next Generation Sequencing (NGS); Which hypervariable region of 16S rRNA gene will appropriate to target most of bacterial taxa...

02 November 2017 542 3 View

Hii, Greeting Everyone i am working on environmental bacterial diversity where i am comparing two different regions of a ecosystem;

from each region sample collection was carried out in triplicate (distance between each replicate will be about 100 meter), now if i will pull the triplicate of each region for further processing...

05 August 2017 885 4 View

How to calculate chlorophyll content in sea water by spectrophotometer method and how to convert mg/m3 to mg/L ?

I am estimating chlorophyll content in sea wate by using spectrophotometer, i already measured Extension at 750, 645, 630 and 665 nm and substrated 750nm reading, but how to calculate further and...

11 August 2016 9,112 0 View