Hello all
I would like to start working with human iPSC cells, and looking for protocols that I can use it for thawing, freezing, regular passaging, and differentiate of this cell line.
Thank s in advance
Hello all, I extracted RNA from my samples and performed RIP-seq. After annotating the genomic regions using R, I obtained promoters, exons, introns, and UTRs. Given that my samples consist of...
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I am doing a RT-qPCR for gene expression analysis of cancer patients, and there are four different groups in the results. The first group that shows over expression of my target gene (both two...
31 May 2024 1,088 0 View
Can anyone interpret why the absorbance (DNS assay method) in alpha amylase assay get increased with increased standard (inhibitor) concentration? it should be decreased with increased standard or...
26 May 2024 4,753 2 View
I attempted a phosphate (apatite) flotation, but the slurry was too thick for the impeller to work properly. I reduced the solid content to 30% for better mixing, but the conditioning still seems...
14 May 2024 4,505 4 View
Hello everyone, recently I am trying to synthesize a dipeptide with a Coumarin derivative of Tryptophan, and the problem is it doesn't contribute in reaction at all. Tryptophan itself in the same...
15 April 2024 6,778 3 View
Dear Authors and Researchers, I'm looking for predictive technology models (PTM) for FinFET including 10nm. Unfortunately, the following webpages do not work...
14 April 2024 4,390 1 View
I did a branch of the DFT calculation to find the stable cell parameter (a) . I didn't get the smooth curve for energy(E) v.s a. There is some point out of concave curve that I had to repeat...
21 March 2024 2,037 2 View
Hello All, I'm doing RNA extraction from cells. I tried both ways by using TRIZOL (with chloroform, and isopropanol) and RNeasy mini kit. When I use TRIZOL, the RNA concentration is high, however...
11 March 2024 9,171 8 View
Why after the adsorption of methyl orange dye by my adsorbent, in addition to the color peak in 464 nm, another peak has appeared in 372nm n in theuv-vis spectrum. It should be noted that this...
10 March 2024 4,167 2 View
Hello All I prepared my library to run for sequencing. I would like to check the quality of my samples through D1000 ScreenTape Assay before add to sequencer. Can anyone help me to interpret my...
10 March 2024 8,764 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,105 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 640 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View