In the dot plot the dead cell population is distributed in the upper left and upper right quadrant. What does it mean when the cells population cloud is extended from the upper right to upper left quadrant?
Dear Rakibul:
Usually, cells go thru different stages during apoptosis; some of them can be detected employing the Annexin V-PI strategy, and they usually are not visualized as totally separated populations. Nevertheless, it is impossible to determine which is your case, without at least knowing how you are performing your experiment and plotting your acquired cells.
You can add a figure to your question, showing how does it looks like.
Good luck!
I understand the difficulty of answering my question. I may come back to you if I manage to attach a picture from one of my results. Thank you very much Matias.
please check also the suggestions and answers of this question
https://www.researchgate.net/post/Whether_PI_Annexin_V-FITC_cells_are_classified_as_LateApoptotic_cells_or_Necrotic_cells1
it would be helpful.
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