Can someone recommend a resource for standard of care drugs for cell lines?

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What are the latest approaches to integrating religious education in today’s curriculum reforms?

The ERATS July 2024 Special Issue is now inviting submissions under the theme ‘Religious Education in a Curriculum Restructuring Era.’ This issue aims to explore the dynamic interplay between...

09 April 2024 8,201 2 View

How effective is community-driven development?

existing community-driven development projects in the Philippines

22 March 2024 8,346 0 View

How to slow down 1165 based lift-off for thermally deposited metals on semiconductors ?

quick summary: I am looking for a way to slow down lift-off (1165 or acetone) for a given material system (changing materials to solve the problem is not possible) This is a relatively general...

08 January 2024 8,955 2 View

Qubit vs Quantus, which is better and why?

Current application is library quantitation for NGS.

05 April 2023 317 1 View

[LAMMPS] How/Where can I get a potential for Germanium-Arsenic and Silicon-Arsenic interaction?

I am planning to simulate the diffusion of As atoms (as dopants) within a growing Ge layer. The system can be pictured as a flux of As and Ge atoms being deposited on a Silicon substrate. The...

29 March 2023 1,458 1 View

Can qPCR products be sequenced in Ampliseq workflow?

We frequently have mixed results for a qPCR assay. We'd like to determine if this is non-specific amplification, or target amplification when the target is low-frequency. DNA tape-station shows...

02 February 2023 6,777 5 View

RQ-PCR, what are these mysterious false positives?

Our lab has an assay to detect fusion transcripts that very frequently results in "false positives." Often our duplicates show mixed results (one positive and one negative), always at a high C(t),...

09 January 2023 2,609 3 View

How is movement on a thrust sheet accommodated form immediately adjacent to, but out side of the sheet?

Every reference paper I have viewed on thrust faulting shows geologic cross section parallel to the direction of movement of the thrust sheet. My question is what do perpendicular cross sections...

27 July 2022 616 0 View

To what extent have customs (customary law) and general principles been influential in the development of international criminal law?

Some scholars argue that the notion of customary law as a source of international law is questionable on the premise that customary law is broad and vague, hence its obscurity is discrepant with...

15 October 2021 2,266 10 View

How did/does customs and general principles form the basis of international criminal law?

The question seeks to explore how international law took/takes customary law and general principles and turns them into general rules.

15 October 2021 1,467 8 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

12 August 2024 862 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

What should Berlin do as a city to become as impactful as London and Paris in World Football?

Please go through my Abstract. I can also share a proposed Thesis Outline.

04 August 2024 2,077 0 View