I have performed antibacterial activity using well plate method, measured the zone of inhibitions at 6 different concentrations, and need to calculate the %ZOI and %RSD to determine ic50.. can someone please guide me?
I really appreciate any help you can provide.
Hi
This YouTube video can help to explain how to calculate CI50.
https://www.youtube.com/watch?v=GgU3KXhKne8&ab_channel=PeterKlappa
Change in absorbance = absorbance at 24hrs - absorbance at 0hrs
% Zone of Inhibition = (change in absorbance for Negative control - change in absorbance for positive control)/ (change in absorbance of negative control) x 100%
% zone of inhibition >50% = MIC ( lowest concentration ratio of the drug to inhibit the growth of microorganisms)
Do measure the inhibition area by mm scale. Devide and multiply it by 100.
Andhare Aishwarya were dealing with well plate method ( 96 well micro titer plate) and it’s measure in ug/ml not mm scale
To calculate the %ZOI, you need to determine the average zone of inhibition for each concentration and express it as a percentage of the maximum zone of inhibition observed in your experiment.
Here are the steps to calculate the %ZOI:
Determine the IC50 value by finding the concentration that corresponds to 50% inhibition. This can be estimated by interpolation or regression analysis.
- Badges
- Science method