i want to know the full protocol of Arabidopsis crossing. how to harvest parents silliques to T3 homozygous selection. on google i can only find the crossing method. but can't find how to handle these plants and next geenration selction.
Not sure if this is what you are looking for, but you can watch the crossing procedure here: https://www.youtube.com/watch?v=QVIPBEwBG5k
the emasculation of flowers for crossing: https://www.youtube.com/watch?v=AIsfhrGkLkc
and the harvesting of siliques generated after crossing: https://www.youtube.com/watch?v=xIjyJCBwyIo&t=26s
Crossing is described in these protocols:
https://www.arabidopsis.org/servlets/TairObject?type=protocol&id=501683738
http://cshprotocols.cshlp.org/content/2006/5/pdb.prot4623.abstract
Gustavo Acevedo-Hernandez thank you so much for the answer. I hope it will help me:)
Gustavo Acevedo-Hernandez thank you so much for the answer. I hope it will help me:)
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