I am working with mice liver injury model where I need to monitor serum ALT and AST levels. Here I have got a problem that I could not proceed with serum seperation of my blood samples (kept in serum vials) and left them at 20degree C without serum seperation for atleast 28hrs. All I want to ask is that what kind of effects will it have upon the results? If the results of ALT and AST levels will be reliable? I can not repeat the test as it was a lonng process so suggest me some troubleshoots as well.
Hello in Punjab,
we did a trial more than 25 years ago (Framstad T., Havre G., Morberg H., Effect of storage, centrifugation and sodium heparinate on clnical chemical analysis of blood constituents from pigs. Nor Vet Tidsskr 1989; 101: 237 - 243) in pigs (!) with blood samples stored both as uncentrifugated blood and stored as serum (and also heparinted plasma) in room temperature for several days. ALAT (ALT) did not change at all in both unsentrifugated blood or in serum for as long as 2 (3) days. ASAT (AST) was a bit different: Stored as serum: almost no difference in 3 days, BUT, stored unsentrifugated 27% higher after 1 day storage, 50 % after 2 days storage . . .
I hope this can be of some help! Best regards from Tore in Norway
It depends on how you had stored the samples. If they were stored at 2-8 C then you can use the sample for calculation of AST and ALT up to 7 days.
I'm afraid that keeping the vials at 20 C could alter serum aminortansferase levels,as quoted by the previoius two colleagues. My suggestoin is to keep the vials (with the blood samples) in a refrigerator (+ 4° C°) up to centrifugation and no longer than 3 days.. Good dluck.
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