What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

How to calculate ECC using HFSS software?

Good morning. Now i am designing MIMO antenna for Ultraband Applications. Can anyone suggest how to plot ECC (Envelope correlation Coefficient) Using HFSS 15 software. Thanks in advance

13 February 2020 5,261 6 View

What is the zeta potential value of deionised or distilled water?

I am getting zeta potential value of about -30 mV for distilled water obtained from a commercial supplier. What it means? What should be the zeta potential value of distilled water? How can I...

10 October 2017 2,791 19 View

Zeta potential for amine functionalized Nanoparticles?

What will be the sign of zeta potential for amine functionalized nanoparticles? The amines are neutral (not protonated). Due to the electron rich character of amine functional group shall I expect...

30 July 2016 9,866 4 View

How to compare more than 50 groups?

i want compare more than 50 groups? SPSS could not performing ANOVA. which test shal i use to compare these groups ? kindly give your suggestions

05 July 2016 3,389 6 View

What is the statistical methods or test to identify first five best groups from several groups?

I am doing some diversity analysis for mineral content. If i want to compare and identify five best groups/samples from 70 samples (for 10 different parameters-independent variables), which...

30 May 2016 956 3 View

Is the zeta potential value valid for amino acid functionalized nanoparticles?

I have modified my carbon nanoparticle surface with amino acids. Now I would have both amine and acid functionalities on the surface of my nanoparticle. The measured zeta potential value is about...

31 December 2015 6,592 10 View

Can anyone suggest literature on Regional Innovation Systems in solar energy in the Indian context?

26 March 2015 5,749 2 View

What can be used as a standard for alpha amylase and glucosidase activity?

Why we should use acarbose as reference for alpha glucosidase activity? Is there any other standard that can be used for this enzyme assay? Can maltose be used as reference for alpha amylase activity?

07 October 2014 1,297 1 View

DTT or BME - which one is better for RNA isolation from 293T cells?

In a Qiagen RNA isolation kit either DTT or BME has to be added to the RLT buffer.

26 March 2014 4,998 4 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View