Studying plasma cell cytokine production in vitro?

01 August 2023 1 9K Report

I am looking to study if plasma cells in one of the models I am working on secrete various levels of cytokines, but I am unsure which stimulation in vitro should be used to look at this? I have used CD40L, TLR agonists and Ig crosslinking in the past to look at B cell specific cytokine production, but not plasma cells.

Does anyone have any experience with this?

Michael Förster

Hi Ellie Alberts

I briefly wanted to reach out concerning your inquiry. There are a couple of options for your experiment.

For murine cells you may consider (tried and tested) IL4 + anti-CD40 + anti-IgM (for BCR crosslinking) for stimulation. You may also consider switching IL4 out or combining it with IL21 (as this seems to work irrespective, if you take mouse or human cells).

For details please see:

Article NCF4 dependent intracellular reactive oxygen species regulat...

Article IL-21 drives expansion and plasma cell differentiation of au...

And for details on plasma cell characterization please consider:

Article MYSM1-dependent checkpoints in B cell lineage differentiatio...

Article Regulation of B Lymphocyte Development by Histone H2A Deubiq...

And for a more general overview with more details, please have a look at this excellent review by Leen Moens and Stuart Tangye. It's a bit older, but key references especially for experimental protocols are included.

Article Cytokine-Mediated Regulation of Plasma Cell Generation: IL-2...

All the best & good luck for your experiments,

Michael

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