What are the methods available to measure the drug-protein interaction?

Hello everyone, I would like to understand the interaction between my drug of interest to a protein (known). Basically, I want to study how strong the interaction is. Is there any way to measure...

10 November 2019 4,636 5 View

How to develop MCF7 mouse xenografts?

Hello everyone, I have two questions, please shed some light on this. I am new to in vivo studies and some may find my questions are trivial. TIA. My first question is, I wanted to develop the...

09 October 2019 4,774 4 View

Could you please help me why my Flag-tagged gene is not expressing?

Hi all, Whenever I try to do immunoblot for FLAG or HA tag, I could not able to see their expression. Do I need to modify my protocol? I am successfully getting images for endogenous expression....

06 July 2018 9,846 10 View

How to get the information about the list of drugs under the clinical trial for a given disease?

Could you please help me to find the information about the list of drugs under the clinical trial for a given disease? I know my question is vague but any closest answer would be helpful for me....

04 May 2018 6,605 5 View

How to determine the intracellular drug concentration?

Hello all, I wanted to know the amount of drug present inside the cell after drug treatment. I checked articles for the same, finally I ended with targeted mass spectroscopy (MRM analysis). But in...

31 December 2017 2,455 3 View

No band for phosphorylated H2AX (gammaH2AX) when I treat with the drug?

Hi all, I have treated the drug that induces DSB to the cells (HEK293T). After 24 h of incubation, I harvested and did Western blot for gH2AX but I did not see any bands. I made 12% gel and...

11 December 2017 1,087 2 View

Is it necessary to synthesis second strand cDNA to amplify the gene of interest?

Dear All, I have synthesised cDNA first strand using SuperScript III (Invitrogen) and would like to amplify my gene of interest for overexpression studies? Is it necessary to synthesis second...

10 November 2017 9,318 11 View

Can someone please recommend me the protocol for the gamma H2AX foci formation assay?

09 October 2017 8,306 8 View

Problems in amplifying my gene of interest from the cDNA ?

Hello Everyone, I am trying to amplifying a gene from the cDNA. I used Superscript III to prepare cDNA and got 1.5 ug/uL concentration. For amplification, I used 50 ng of cDNA template. But I...

09 October 2017 3,712 6 View

How to avoid dislodging the cells while changing fresh media?

Please help me with this. I'm working on cell viability assay. I used to seed the cells a day before adding the drug. My observations are: few cells are coming out while aspirating the old...

08 September 2017 4,231 8 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View