20 Questions 27 Answers 0 Followers
Questions related from Arun Pandian Chandrasekaran
Hello everyone, I would like to understand the interaction between my drug of interest to a protein (known). Basically, I want to study how strong the interaction is. Is there any way to measure...
11 November 2019 4,741 5 View
Hello everyone, I have two questions, please shed some light on this. I am new to in vivo studies and some may find my questions are trivial. TIA. My first question is, I wanted to develop the...
10 October 2019 4,892 4 View
Hi all, Whenever I try to do immunoblot for FLAG or HA tag, I could not able to see their expression. Do I need to modify my protocol? I am successfully getting images for endogenous expression....
07 July 2018 9,951 10 View
Hello everyone, I have an experience on CRISPR/Cas9-mediated genome editing. So far, I have designed more than 200 guides targeting different human genes and their efficiency have been validated...
07 July 2018 4,421 2 View
Could you please help me to find the information about the list of drugs under the clinical trial for a given disease? I know my question is vague but any closest answer would be helpful for me....
05 May 2018 6,698 5 View
Hello all, I wanted to know the amount of drug present inside the cell after drug treatment. I checked articles for the same, finally I ended with targeted mass spectroscopy (MRM analysis). But in...
01 January 2018 2,557 3 View
Hi all, I have treated the drug that induces DSB to the cells (HEK293T). After 24 h of incubation, I harvested and did Western blot for gH2AX but I did not see any bands. I made 12% gel and...
12 December 2017 1,189 2 View
Dear All, I have synthesised cDNA first strand using SuperScript III (Invitrogen) and would like to amplify my gene of interest for overexpression studies? Is it necessary to synthesis second...
11 November 2017 9,425 11 View
Hello Everyone, I am trying to amplifying a gene from the cDNA. I used Superscript III to prepare cDNA and got 1.5 ug/uL concentration. For amplification, I used 50 ng of cDNA template. But I...
10 October 2017 3,840 6 View
Can someone please recommend me the protocol for the gamma H2AX foci formation assay? Many thanks in advance, Arun.
10 October 2017 8,511 8 View
Please help me with this. I'm working on cell viability assay. I used to seed the cells a day before adding the drug. My observations are: few cells are coming out while aspirating the old...
09 September 2017 4,335 8 View
Hello, everyone. I'm trying to subclone a 5 kb gene to a vector (approx. 5 kb). Initially, I designed the primers which have restriction enzymes overhang (XhoI and XbaI). I amplified my gene using...
08 August 2017 6,444 5 View
Basically, heterochromatin is a tightly bounded DNA region. Due to this reason, polymerases cannot access these regions and therefore no transcription. Does that mean heterochromatin region is...
06 June 2016 3,794 7 View
I was wondering how a restriction enzyme creates sticky end (or blunt end)? What makes it cut so? Thanks in advance.
06 June 2016 9,203 2 View
I'm looking to get full version of EndNote X6. Before that I wanted to be well-versed in this tool. I have searched for tutorials but ended up with none. It would be grateful if someone send...
02 February 2015 8,293 1 View
I have seen some methods in web but it seems to be difficult. I am a beginner to this kind of work. Any suggestions from your side would be very helpful. Thanks in advance.
01 January 2015 7,393 7 View
I'm working on bioethanol production. After pretreatment, I usually used to check SEM and TEM to study its morphological changes. And I will check how far the complex structure destructed in...
01 January 2015 9,121 14 View
I'm raising this question on the behalf of my colleague. She is working on nano composite material synthesis.She wanted to check anti-microbial activity of the composite. Whenever, she...
01 January 2015 7,408 5 View
I have results for FTIR, SEM, UV and XRD of my sample. As a beginner, I really need of books which helps me to interpret the characterization results. Some basic books to understand the concept of...
12 December 2014 8,128 4 View
Some people use mL rather than ml? What is the correct symbol to express milliliter?
11 November 2014 5,247 3 View
