How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

Anyone familiar with antioxidant studies on rats' brain homogenate?

I am looking for a skilled personnel in Kuwait University that is familiar with antioxidant studies using ELISA kit or other techniques on rat's brain homogenate, particularly the hippocampal and...

04 November 2020 5,481 1 View

In which solvent should I reconstitute mitoTEMPO?

Dear colleagues, I was wondering which solvent should I use to reconstitute mitoTEMPO, I've seen I could use water or DMSO but I'm not sure about the stability of the compounds in the respective...

18 May 2020 2,882 3 View

What are primers shall i use to detect superoxide dismutase, catalase and glutathione S teransferase through RT-PCR technique for Liriomyza trifolii?

I need to compare between different treatments through real-time PCR technique upon Liriomyza trifolli (insect pest) genome, but I could not know what is the sequence of primers shall I use to...

28 April 2020 4,161 1 View

Does anyone has simplified methods for Superoxide dismutase (SOD) assay?

I would like to exam enzymatic antioxidant for some cinnamic acid derivatives compounds, so I am planning to use superoxide dismutase assay, so if you have a clear method for this examination...

25 December 2019 9,523 8 View

I'm having problems with Superoxid Dismutase assay. Is there anything I can change in the metodology?

I've been studying plant metabolism and in my lab we ar used to measure superoxid dismutase activity using the methodology of Giannopolitis e Ries, (1977), using the nitrotetrazolium blue...

17 December 2019 7,272 1 View

I'm having problems with Superoxid Dismutase assay. Is there anything I can change in the metodology?

I've been studying plant metabolism and in my lab we ar used to measure superoxid dismutase activity using the methodology of Giannopolitis e Ries, (1977), using the nitrotetrazolium blue...

11 December 2019 4,136 1 View

Does anyone has simplified methods for Superoxide dismutase (SOD) assay?

I would like to exam enzymatic antioxidant for some cinnamic acid derivatives compounds, so I am planning to use superoxide dismutase assay, so if you have a clear method for this examination...

11 December 2019 6,572 6 View

How much free radicals can reduce yield in desired conditions for growth?

The presence of free radicals in photosynthetic and respiratory systems is inevitable, even in optimal condition for growth. In these conditions, How much free radicals can be loss in yield?

02 March 2019 4,564 9 View

How can we find initial velocity if substrate concentration(S1,S2,S3,S4,S5) and absorbance are given incase of superoxide dismutase enzyme?

It includes application of michelis menton equation but i don't know how to find velocity if absorbance values are given. I used pyrogallol as substrate incase of superoxide dismutase enzyme which...

01 February 2019 4,144 5 View

How to prepare 75 uM nitro blue tetrazolium HCl prior to superoxide dismutase (SOD) assay?

Does anyone know how to prepare nitro blue tetrazolium solution at 75 uM? Some websites suggested 70 % dimethlformamide (DMF) solution or ethnaol... I wonder the solvent can be used or not?...

23 January 2019 5,474 7 View