Lalrinzuali Khawlhring

11 Questions 27 Answers 0 Followers

Questions related from Lalrinzuali Khawlhring

Can someone help with the daily clean regime of flow cytometer?

Hi, I'm just starting out on flow cytometry. Regarding the daily clean option, the instrument engineer told that we need to run the daily clean option + all the four fluidics options only once a...

15 November 2022 6,210 3 View

What is validation of data in HPLC?

In HPLC, what is the validation of data? How to do it?

13 November 2017 6,495 4 View

Why does hpv replicate only on squamous epithelium??

i have read an article that state that hpv can replicate only on stratified squamous epithelium. why is it so? thank you

27 April 2016 9,055 2 View

How do i identify mf7, hela and hepg2?

i would like to know how to identify mcf7, hela and hepg2 cell line under microscope, whats their main difference in morphology?? thank you

25 April 2016 1,249 2 View

How to calculate SOD?

i have used the following protocol for SOD in a 5% cell homogenate.. Sample 100 ulPhenazene methosulfate-186 mM- 100 ulNitroblue tetrazolium -3.0 mM -300 ulNADH -780 mM- 200 ulIncubate for 90...

08 February 2016 8,860 16 View

What does it mean when treatment of tumor cell line using plant extract gives biphasic curve?

i have treated a tumor cell line using a plant extract and terminated the treatment at different times (eg: 1 hour, 2 hours, 4 hrs and so on) and estimated some enzyme activities and observed that...

03 February 2016 3,397 3 View

How to store hela cell homogenates?

i have 102 samples and i have to estimate protein, gsh, lipid peroxidation, gst, gshpx, catalase and sod..... it will take weeks to finish all these assays, so, after making cell homogenates in...

20 January 2016 3,090 2 View

My MTT reading is fluctuating.. can someone help me?

i have performed MTT as per Mosmann T on HepG2 cell line using different concentrations of plant extracts (1-90 ug/ml). The absorbance of all the treated groups are reduced to about half as...

01 November 2015 4,839 9 View

Can someone help me regarding drug preparation for MTT assay?

i want to perform mtt assay, but am having a bit of confusion regarding drug preparation. i want to add 10 microliter of my extract in a 100 microliter cells containing 10,000 cells. suppose i...

15 October 2015 3,153 3 View

Hi, is there any facility in india where I can send my plant extracts for in vitro anticancer activity tests?

i need mtt, clonogenic, comet assay etc.

28 July 2015 7,342 6 View

Can someone please explain the mechanism of superoxide dismutase activity assay?

I am currently working on enzyme assays on tumor cell, n the protocol i use for SOD is incubating sample, PMS, NBT and NADH for 90secs at 30C and adding acetic acid + butanol + absorbance at 560...

17 November 2014 7,453 7 View