How to segregate electrode-dielectric and dielctric-dielctric interface polarization after Capacitance VS Freq. measurement using impedance analyzer?

After measuring the capacitance vs frequency for my MIM capacitor consisting of alternate layers of TiO2 &Al2O3 , there is a rise in capacitance value of ~ 20-160 nF at 100 hz, starting to...

17 December 2020 7,786 3 View

How to start with Principal Component Analysis?

I want to carry out PCA on a set of chemical data, some of them in oxide form and some in elemental form. The oxides are in percentage and the elements are in ppm. I have understood that, the...

14 December 2020 2,201 7 View

Are women working in corporate world are at more risk due to pandemic Covid-19?

A recent report shows that working women in corporate sector are likely to quit their jobs or downshifting their careers due to challenges faced by Covid-19. It is said that may be this is due...

06 November 2020 588 19 View

According to WFP( World Food Program me) access to food is problem in many South Asian Countries . How to solve it??

This is the 21st century. We talk about going to Mars. We got satellites all over the place. But at the same time we have the people dying because they don't have enough food to eat which...

02 November 2020 9,997 4 View

Is it time to re-open schools and colleges?

It has been more than 8 months that Pandemic Covid-19 entered into our life and we are still struggling. With 5.0 unlocking, many things become normal but one thing which is yet to be back to...

01 November 2020 7,105 55 View

Is it true that happy people live longer??

A study on Biological Psychiatry found that aging is successfully linked with a choice to live happy and productive life. Any one who limits his vision to memories of yesterday is already dead -...

21 October 2020 6,503 19 View

Is on line violence against women emerging as a social evil?

Recent study shows that more than 50% women face abuse on social media - from unwanted friend request to indecent messages to peculiar marriage proposals, women face harassment on social media...

14 October 2020 1,668 18 View

Can pandemic mess with our memories?

Research says - pandemic and depression, anxiety and stress are linked and these mental health problems can lead to false memories. Also. it has been found that emotions which are mostly...

07 October 2020 4,221 26 View

What is holistic approach to Higher Education?

Having excellent faculty is one important way to bring quality change in Higher Education. Emphasizing on much needed flexibility, multi-disciplinary creativity, and critical thinking and also...

24 September 2020 4,230 45 View

Can anyone share publication of Seck HA (1971a, b)?

24 August 2020 2,575 2 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

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Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

What are some common oxidants used to oxidize iron sulfur clusters in proteins for techniques like EPR?

I have used potassium ferricyanide (which is likely the most common) extensively in the past, but I am curious to know if there are other oxidants that work well. I need to generate the oxidized...

29 January 2021 9,803 4 View

Papain Enzyme Assay...?

Hi all, I would like to know how can I estimate the activity of the enzyme, papain, which is used in reactions as crude latex extract of papaya. Please share some procedures if anybody having...

26 January 2021 569 4 View

Where can I find plants pyr-GC-MS data?

25 January 2021 5,403 2 View

Recombinant restriction enzyme: how strong should the corresponding methyltransferase expression level be?

Hello. We are planning expression of a restriction enzyme in E. coli, and wondering how much of the corresponding methyltransferase is needed to be expressed in order to protect the host genome...

23 January 2021 2,096 4 View