Can I use triphenyl tetrazolium chloride (TTC) instead of nitroblue tetrazolium (NBT) to estimate SOD activity?

13 February 2024 1 5K Report

Both TTC and NBT yield formazan when interacting with oxides. The superoxide dismutase assay requires NBT, which reacts with oxide to form formazan. TTC also produces Formazan when it interacts with oxygen. So, can I use triphenyl tetrazolium chloride (TTC) instead of nitroblue tetrazolium (NBT) to estimate SOD activity?

P.F. Zabrodskii

I think you can!

https://en.wikipedia.org/wiki/Tetrazolium_chloride

https://en.wikipedia.org/wiki/Nitro_blue_tetrazolium_chloride

Badges
Science topic

More Aneeq ur Rehman's questions See All

Data balancing before test/train split or only training data balancing. which is correct?

While reviewing technical articles, I encounter some papers where authors have used data balancing before splitting test and train (on full data), and some only balanced training data and let the...

04 February 2021 1,446 0 View

Mental health of COVID-19 Patients?

We want to conduct a study on the mental health and psychological problems in COVID-19 patients and would measure it in terms of Anxiety by Beck Anxiety Inventory, Depression by Patient Health...

02 February 2021 6,401 6 View

Is PV array not available in MATLAb 2020a simulink?

I have MATLAB 2020a. PV array is not present in the simulink library. Can anyone please explain if the name of module has been changed or is it not available anymore? Is there any way to add PV...

24 January 2021 7,962 5 View

What counts as publication in scholarly journals?

Hello Seniors! Please guide me, what counts as a publication in a scholarly journal? e.g. book reviews etc.

24 December 2020 10,038 1 View

Does book Review in scholarly journals counts as a publication?

Does book Reviews written for scholarly journals or in a magazine count as a publication?

24 December 2020 2,265 3 View

Does Preprint research article count as a publication?

Hello friends, my article has been published in AIJRPreprint online and it is also available on Google Scholar. Would it be count as a publication in my CV? Thanks

23 December 2020 3,332 11 View

In ELISA should we use p-nitrophenyl phosphate (pNPP) substrate mixed in distilled water or we should mix it in Diethanolamine buffer?

We are performing an ELISA experiment to determine the binding of Complement antibodies against P. aeruginosa. We have pNPP substrate (Thermo Scientific cat # 34045) instead of pNPP tablets. We...

22 December 2020 2,020 3 View

Why so different behavior of gravitational force ??

If a lift is falling freely with "g", the person inside will be experiencing or feeling No force, and he becomes weightless, means force of gravity is constantly acting and producing "g", but the...

06 December 2020 6,721 1 View

What will be the form of vander wall and DL term in DLVO theory for cellouse nanofibrils?

Hello I am using C-CNF in DI water and dip a flat polymer in the solution to form CNF coatings on the polymer. since DLVO didnt account for size and shape of the molecules to cause forces flat...

21 November 2020 2,308 4 View

What the weak diffraction spots indicate?

These diffraction patterns were obtained for TbCu7 type SmCo7 melt spun ribbons. XRD patterns shown that there is only one phase in these alloys. I do not understand what the weak diffraction...

17 November 2020 4,937 4 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View