Asneel Ashesh Lal

Analyzing antioxidants in livestock, particularly dairy cows and goats, involves studying key enzymes such as Superoxide dismutase (SOD), Glutathione peroxidase (GPx), and Malondialdehyde (MDA). For Superoxide dismutase analysis, a common method is the inhibition of epinephrine autoxidation, measuring the rate of inhibition spectrophotometrically. Glutathione peroxidase can be assayed by monitoring NADPH oxidation in the presence of glutathione and hydrogen peroxide. Lastly, Malondialdehyde, an indicator of lipid peroxidation, is often measured using the thiobarbituric acid reactive substances (TBARS) assay. For SOD, prepare a reaction mixture with epinephrine and enzyme extract, measure absorbance change at 480 nm. For GPx, incubate sample with glutathione, hydrogen peroxide, and NADPH, measuring absorbance change at 340 nm. For MDA, react sample with thiobarbituric acid, heating and then measuring absorbance at 532 nm. Detailed steps and reagent concentrations can vary, so it's advisable to refer to established protocols or literature for precise methodologies tailored to your specific laboratory conditions and equipment. Always ensure the accuracy and reproducibility of your results through appropriate controls and validation steps.

Rizwan Mehmood Bhatti do you have SOP for the above antioxidant tests? If possible, can you share via email?

Asneel Ashesh Lal

Materials and Equipment:

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References:Refer to established protocols or scientific literature for detailed methodologies.

Approval:This SOP has been reviewed and approved.

Revision History:

• Version 1.0: [Date] - Initial SOP
• [Any subsequent revisions with dates and reasons]

Note: Customize this SOP according to your specific laboratory conditions, equipment, and reagents. Always adhere to safety guidelines and ethical standards.