Translating two biomarkers to the same scale??

Hello researchers, I was wondering if anyone had experience in the following: I would like to translate two separate continuous biomarkers (A and B), that are mechanistically linked to each...

25 January 2021 2,107 3 View

What would cause different shape DMA loss modulus curve in different batches of PC+ABS?

I have some DMA curves of PC+ABS from different batches. The PC loss modulus peak is fairly consistent but one batch has a peak value 10degC lower than the rest. Another peaks at the normal value,...

06 December 2020 809 2 View

Nature of Racism?

I am writing a scholarly advocacy article - the question is: "What is the problem with using an historically specific example of racism as a template for understanding the multifaceted nature of...

29 May 2020 7,848 22 View

Phosphomimetic Peptides & Phospho-Specific Antibodies?

I am performing experiments using synthetic cell-penetrating peptides in acute hippocampal slices. Our lab has verified the NLS-mediated nuclear delivery of a number of such peptides in the region...

27 December 2019 7,914 2 View

What is the recommended fixation time of 400 um hippocampal slices in 4% PFA?

I am currently processing 400 um hippocampal slices immunohistochemically. I have not had too much trouble with my images and antibody binding. That said, I am aware that over fixation may...

22 June 2018 9,560 5 View

How long does Apex Taq RED Master Mix, 2X remain viable after opening?

The manufacturer's data sheet says the unopened vial lasts for 2 years at -20 C and that the mix is stable at 4 C for at least six months. The dye we are using has been opened and has been kept at...

10 June 2018 3,902 3 View

Is agitation necessary during IHC antibody incubation for 400 um hippocampal slices?

I am processing 400 um hippocampal slices immunohistochemically. I am incubating the slices in the primary antibody solution overnight at 4 degrees C and the secondary antibody and nucleic acid...

09 June 2018 9,562 3 View

Is it normal for signal intensity to fluctuate during chemiluminescent detection with Versadoc?

I've noticed this strange phenomenon where my signal intensity will change from picture to picture over the course of my exposure. For example: I will expose membrane to ECL for 2 min, signal...

13 February 2018 1,529 2 View

How to use McNemar's test to compare accuracy of classifications?

Hi, I have seen various studies use McNemar's test to statistically test the accuracy of several classifications. I have six classifications, produced using three different classifiers (SAM, SVM,...

19 September 2017 5,034 4 View

What's a typical response rate for an on-line survey?. Have you got a good UK based reference? (Or any reference at all?)

I'm especially interested in response rates to on-line validation studies.

26 October 2014 3,842 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View