15 May 2014 1 7K Report

I am pursuing the identification and development of technologies/innovations for smallholder farmers in sub-Saharan Africa to adapt to and mitigate the effects of climate change with respect to rainfall amounts, distribution and reliability.

More Spencer Muthoka's questions See All
Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

Best way to separate lipids, nucleotides, and proteins in cell culture media?

Hello everyone. I need to be able to take cell culture media that has already been on cells and essentially make three different solutions from it: lipids, nucleotides, and proteins. The eventual...

29 April 2024 2,817 0 View

Have you any suggestions on how to increase engagement with a proposed idea?

I am attempting to describe the creation of a Research Institution employing a totally different perspective than I have experienced throughout my years in academia. Thus far, the engagement has...

09 January 2024 801 0 View

Solubility issues for an injectable?

I am trying to solubilize a peptide:oligonucleotide conjugate for intra-peritoneal injection into a mouse. At low concentrations, the mixture dissolves nicely in either H2O or saline, however at...

04 May 2023 8,502 1 View

Manual calculation of interaction p-value?

Hello, I was wondering if there was a way to calculate interaction p-value by hand using estimates from a published article that does not report the p-value?? For instance, if the HR (95% CI)...

04 April 2023 5,515 1 View

Regarding the Cattell–Horn–Carroll theory of intelligence, what causal claim does the theory make about the relationship between IQ and abilities?

I'd love your help understanding this question. I'm confused about what causal claims the Cattell–Horn–Carroll (CHC) theory of intelligence makes. As you likely know, it defines a hierarchy of...

04 February 2023 1,681 1 View

I want to plate 1000x more phage than bacteria cells - how do I do this?

I have the phage sample titer and the bacteria colony forming units (CFU) - but how can I calculate how much of each I want to plate knowing I need to plate 1000x more phage than bacteria cells?...

10 August 2022 4,886 2 View

What to dissolve 4-methylumbelliferone palmitate in for biological assay?

I need to eventually combine this solution with a .1M sodium acetate buffer with triton X. Eventually, this will be mixed with lysates and read for the presence of 4-MU fluorescence. I started out...

11 November 2021 7,887 1 View

How long can CHO cells survive for on a dry plate?

tldr: I have a dry well of a 24 well plate that did have suspension CHO-K1 cells in it up until about 5 days ago when I upscaled them. The plate has been kept in a humidified incubator since (a...

03 August 2021 417 3 View

Non-specific staining in IHC?

I am trying to stain for a receptor in feline kidneys (not been documented before), with the eventual aim to quantify it. I have tried a few antibodies with similar unsatisfactory results...

01 August 2021 7,949 2 View

Similar questions and discussions
Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close